摘要
[目的]希金斯炭疽菌可以侵染十字花科诸多植物引起炭疽病,给各国农业生产造成了巨大经济损失。对该菌候选效应分子进行预测,并对其特征进行明确。[方法]利用Signal P、Prot Comp等程序对该菌中分泌蛋白进行找寻,并对分泌蛋白所具有的氨基酸大小、信号肽长度以及理化性质等进行分析。结合已经报道的真菌效应分子具有的典型特征,明确该菌存在的候选效应分子数量及特征。[结果]希金斯炭疽菌含有658个分泌蛋白,氨基酸长度集中于50~300 aa之间;信号肽长度以17~21个aa的序列最为集中;信号肽切割位点属于A-X-A类型;分泌蛋白在分子量、等电点、不稳定系数等方面均存在差异,但大多数分泌蛋白属于亲水性蛋白。其中,预测功能的蛋白为290个,其功能较多的集中于酶类,包括脂肪酶、α-L-鼠李糖苷酶、果胶酯酶等。依据候选效应分子典型特征,明确该菌中含有388个候选效应分子。[结论]通过上述生物信息学分析方法,结合真菌效应分子典型特征,有效地实现了希金斯炭疽菌候选效应分子的预测,其信号肽切割位点类型与其他已经报道的致病疫霉、粗糙脉孢霉等分泌蛋白信号肽切割位点一致,分泌蛋白功能涉及较多的酶类,也与其他已经报道的病菌分泌蛋白功能相类似。
[ Objective] Colletotrichum higginsanum can infect many cruciferous plants ,causing tremendous economic losses in agricultural production in many countries. Effector proteins play a more important role during the pathogenic process including infection,colonization,and expansion of plant pathogens. To identify the candidate effector protein from C. higginsanum, and clearly it＇s characteristic. [ Methods] 16 172 protein sequences in C. higginsanum was analyzed to find the secreted protein u- sing the program including SignalP, ProtComp, TMHMM, big -PI Fungal Predictor and TargetP. Meanwhile, the distribution of amino acids, the length of signal peptide, the signal peptide cleavage site as well as physical and chemical properties of secreted protein was analyzed. [ Results]658 secrete proteins were found in C. higginsanum. The length of amino acids of secreted pro- tein was concentrated between 50 to 300 aa; the length of signal peptide was concentrated between 17 to 21 aa; signal peptide cleavage site belongs to AXA type; there are differences in terms of molecular weight, isoelectric point, the instability index in secreted proteins, but which belongs to the hydrophilic protein. Further, there are 290 secreted proteins with predictive function, which are concentrated on enzymes,including fipase, alpha - L -rhamnosidase, pectinesterase. And 388 candidate effectors pro- tein were identified. [ Conclusion]Through the above bioinformatics analysis,and based the character of effector in fungi, the predicted candidate effector proteins can effectively achieve in C. higginsanum, and the type of signal peptide cleavage site have consistent with other secreted proteins from Phytophthora infestans, Neurospora crassa, and its function have similar with other reported secreted protein from pathogen.
出处
《生物技术》
CAS
CSCD
北大核心
2015年第6期546-551,共6页
Biotechnology
基金
国家自然科学基金项目("钩状木霉与枯草芽孢杆菌对核桃炭疽病的协同生防作用机制研究"
No.31560211)
云南省森林灾害预警与控制重点实验室开放基金项目("钩状木霉与枯草芽孢杆菌对核桃炭疽病的协同控制技术研究"
No.ZK150004)
云南省优势特色重点学科生物学一级学科建设项目(No.50097505)
云南省高校林下生物资源保护及利用科技创新团队(No.2014015)
云南省教育厅科学研究基金项目("核桃炭疽病菌Gα-GTP的效应分子在生长发育与致病过程中的功能研究"
No.2014Y330)资助
关键词
希金斯炭疽菌
候选效应分子
信号肽
预测程序
Colletotrichum higginsanum, candidate effector protein, signal peptide, prediction algorithm
