摘要
目的建立广西地区新生儿出生24h内T淋巴细胞亚群与IgG、IgA、IgM生物参考区。方法评估与记录产妇和新生儿病史与临床资料，于新生儿出生24h内采血，0．5h内送检，分析数据，计算生物参考区。新生儿分为早期早产儿、晚期早产儿、足月儿3组，各110例，产妇分为使用激素组与未使用激素组，采用SPSS17．0统计学软件对各组及不同性别新生儿进行比较。结果早期早产儿、晚期早产儿、足月儿双侧95％可信区间参考值分别为CD3^＋：52．07—88．9g/L，58．16—90．42g/L，56．15—95．67g/L；CD4^＋：25．20～59．26g/L，31．27—72．91g／L，28．44—82．66g／L；CD8’：7．30～36．26g/L，9．13～38．49g/L，11．09～48．99g／L；CD4^＋／CD8^＋：0．34～4．58，0．34～4．58．0．32～3．80：CD19^＋：3．95～27．59g／L，4．04～30．94g／L，4．08—38．70g/L；NK细胞：1．34～6．64g／L，2．88～8．92g／L，3．07～9．35g／L；IgA：0．0004～0．0396g／L，0．0000～0．0690g/L，0．0000～0．0690g／L；IgM：0．0016～0．1584g／L，0．0200～0．1400g／L，0．0200—0．4200g／L；IgG：3．22—10．98g／L，1．10—14．62g/L，5．00～13．66g／L。其中使用激素组：晚期早产儿CD8^＋10．35～40．33g／L、NK细胞3．10～9．46g／L，足月儿NK细胞6．60～9．50g／L；未使用激素组：晚期早产儿CD8^＋8．42～34．96g/L、NK细胞2．94～7．80g／L，足月儿NK细胞2．98～8．94g／L；不同性别之间男：晚期早产儿CD8^＋8．26～35．66g/L，足月儿CD3^＋ 51．90～92．94g／L；女：晚期早产儿CD8^＋ 11．08～40．68g/L，足月儿CD3^＋ 61．10～96．14g/L。结论广西地区新生儿出生24h内T淋巴细胞亚群与IgG、IgA、IgM检测值离散度较大，且不同胎龄新生儿检测水平不同，母亲妊娠期间使用激素、性别对新生儿免疫功能有一定影响。
Objective To establish biological reference intervals of neonatal T lymphocyte subsets and IgG, IgA, IgM levels in 24 - hour newborns in Guangxi. Methods Maternal history and neonatal clinical data were evalua- ted and recorded. Venous blood samplings were collected within 24 hours of birth and were sent for testing in half an hour. The neonates were divided into the early-preterm, the late- preterm and the term neonates group, 110 cases for each group. The parturients were divided into Dexamethasone treatment group and without Dexamethasone treatment group. Data in neonates and the parturients and the sex were analyzed by SPSS 17.0 software and the biological reference values were calculated. Results The two - sided reference intervals of 95% in the early - preterm group, the late - preterm group and the term neonates group were as follows ： CD3 ＋ ： 52.07 - 88.92 g/L, 58. 16 - 90.42 g/L, 56. 15 -95.67 g/L;CD4 ^＋ ：25.20 -59.26 g/L,31.27 - 72. 91 g/L,28.44 - 82.66 g/L; CD8^＋ ：7.30 - 36.26 g/L, 9.13 -38.49 g/L,11.09 -48.99 g/L;CD4 ＋/CD8 ^＋ ：0.34 -4.58,0.34 -4.58,0.32 -3.80; CO19 ^＋ ：3.95 -27.59 g/L,4.04 - 30.94 g/L, 4.08 - 38.70 g/L; NK cell ： 1.34 - 6.64 g/L, 2.88 - 8.92 g/L, 3.07 - 9.35 g/L; IgA ： 0. 000 4 - 0. 039 6 g/L,0. 000 0 - 0. 069 0 g/L, 0. 000 0 - 0. 069 0 g,/L; IgM ： 0. 001 6 - 0. 158 4 g/L, 0. 020 0 - 0. 140 0 g/L,0. 020 0 -0.420 0 g/L;IgG ：3.22 - 10.98 g/L, 1.10 - 14.62 g/L ,5.00 - 13.66 g/L. Moreover the cases with Dexamethasone treatment were as follows：the late -preterm infants CD8 ^＋ 10.35 -40.33 g/L, NK 3.10 - 9.46 g/L,term NK 6.60 -9.50 g/L; those in without Dexamethasone treatment： the late -preterm infants CD8^＋ 8.42 - 34.96 g/L, NK 2.94 - 7.80 g/L, term NK 2.98 - 8.94 g/L; according to gender, the males in the late - preterm infants CD8 ^＋ 8.26 -35.66 g/L,term CD3 ^＋ 51.90 -92.94 g/L; females in the late- preterm infants CD8 ^＋ 11.08 -40.68 g/L, term CD3 ^＋ 61.10 -96. 14 g/L. Conclusions Testing values of neonatal T lymphocyte subsets and IgG, IgA, IgM levels in 24- hour newborns in Guangxi disperse largely and show some differences among the early - preterm neonates, the late - preterm neonates and the term neonates, and maternal Dexamethasone treatment during pregnancy and gender play a role in neonatal immunity.
出处
《中华实用儿科临床杂志》
CSCD
北大核心
2016年第2期116-119,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
吴阶平医学基金会临床科研专项资助基金（320.6750.14171）
广西壮族自治区卫生厅重点课题（重2012022）
关键词
婴儿
新生
免疫功能
T淋巴细胞亚群
免疫球蛋白
Infant, newborn
Immunologic function
T lymphocyte subsets
Immunoglubulin
