摘要
Actin is a highly conserved cytoskeletal protein that is found in essentially all eukaryotic cells,which plays a paramount role in several basic functions of the organism, such as the maintenance of cellshape, cell division, cell mobility and muscle contraction. However, little is known about actin gene family inChinese amphioxus (Branchiostoma belcheri). Here we systemically analyzed the actin genes family inBranchiostoma belched and found that amphioxus contains 33 actin genes. These genes have undergoneextensive expansion through tandem duplications by phylogenetic analysis. In addition, we also providedevidence indicating that actin genes have divergent functions by specializing their EST data in both Bran-chiostoma belched and Branchiostoma florida. Our results provided an alternative explanation for the evolu-tion of actin genes, and gave new insights into their functional roles.
肌动蛋白基因家族是一种普遍存在于真核生物中高度保守的细胞骨架蛋白,它们在生物机体中发挥着重要的角色,比如细胞形状的维持、细胞分裂、细胞迁移以及肌肉收缩等等。然而,目前对于肌动蛋白基因家族在白氏文昌鱼中的研究尚且不多。本文利用生物信息学软件分析了该基因家族在白氏文昌鱼中的分布以及表达情况,研究发现在白氏文昌鱼中存在33个肌动蛋白基因。系统进化分析进一步显示,这些基因在进化过程中通过串联重复发生了倍增。此外,通过研究基因的EST数据发现两种文昌鱼(白氏文昌鱼和佛罗里达文昌鱼)中的肌动蛋白基因发生了功能分化。研究结果揭示了肌动蛋白基因的进化以及它们在脊索动物发育中功能角色的演变。
出处
《科学教育与博物馆》
2016年第1期28-36,共9页
Science Education and Museums
