摘要
目的：提取红砂叶片和长叶红砂叶片中总黄酮并测定含量。方法：采用Na NO2-Al（NO3）3-Na OH法显色,芦丁为对照,紫外-可见分光光度法在510nm处测定。结果：红砂叶片和长叶红砂叶片中总黄酮的含量分别为19.72mg/g和17.69mg/g。回归方程为A=12.935c＋0.0003,相关系数r为0.9995,在0~60μg/m L范围内线性关系良好,回收率为95.83%~104.2%（n=9）,精密度、稳定性、重现性良好。结论：该法简便,准确,可作为红砂叶片和长叶红砂叶片中总黄酮的含量测定方法。
Objective：To extract and determine the content of total flavonoids in the leaves of Reaumuria Soongorica and Reaumuria trigyna. Methods：Total flavonoids of the samples were determined at 510 nm by UV spectrophotometry with rutin as standard control.Results： The contents of total flavonoids in the leaves of Reaumuria Soongorica and Reaumuria trigyna were 19.72mg/g and 17.69mg/g.The regressive equation was A =12.935 c ＋0.0003（r =0.9995）,which had a good linear relationship in the range of 0 ~60μg/m L.The recovery were 95.83% ~104.2%（n =9）. Conclusion： the method is simple,rapid,accurate and which could be used as a means to measure total content of flavonoids in the Reaumurid Soongorica and Reaumuria trigyna.
出处
《北方药学》
2016年第2期3-4,共2页
Journal of North Pharmacy
基金
国家自然科学基金(81102760)资助
关键词
红砂叶片
长叶红砂叶片
总黄酮
含量测定
Reamuria Soongorica
Reaumuria trigyna
Total Flavonoids
Determination of Content
