摘要
为了探讨苜蓿(Medicago sativa)对蚜虫取食防御反应的生化机制,本研究通过室内人工接虫,研究了豌豆蚜(Acyrthosiphon pisum)取食后苜蓿体内防御性酶活性、营养物质含量的动态变化。结果表明,豌豆蚜为害7 d后,与低抗品种(惊喜、WL343HQ和德宝)相比,抗虫品种(三得利、MF4020、皇后、皇冠和SR4030)的苜蓿幼苗苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)、过氧化物酶(POD)及可溶性糖活性变化总体上显著增高(P<0.01),多酚氧化酶(PPO)和过氧化氢酶(CAT)活性变化显著降低(P<0.01),中抗品种(柏拉图、甘农4号、先行者和中苜3号)介于其间,可溶性蛋白质含量变化量无明显规律。
In order to investigate the biochemical mechanisms of alfalfa responses to pest aphid,the defensive enzyme activities and nutrients and their dynamic changes were studied in alfalfa varieties with different aphid resistance which were damaged by Acyrthosiphon pisum. The results showed that the activities of L-phenylalanin ammo-nialyase（ PAL）,superoxide dismutase（ SOD）,peroxidase（ POD） and soluble sugar significantly increased（ P〈0. 01） within seven days of A. pisum damaged,whereas,the activities of polyphenol oxidase（ PPO） and catalase（ CAT） significantly declined（ P〈0. 01） in aphid resistance alfalfa varieties（ Santory,MF4020,Queen,Crown,SR4030） compared with low resistance alfalfa varieties（ Surprising,WL343 HQ,Derby）,middle resistance alfalfa varieties（ Platon,Gannong No. 4,Pioneer,Zhongmu No. 3） are somewhere in between. The soluble protein content changed without regular pattern.
出处
《草业科学》
CAS
CSCD
北大核心
2016年第1期144-152,共9页
Pratacultural Science
基金
国家牧草产业体系--盐池综合试验站(CARS-35-42)
宁夏回族自治区农作物重大育种专项--苜蓿新品种选育(2014NYYZ03)
关键词
苜蓿品种
豌豆蚜
危害胁迫
防御性酶
营养物质
alfalfa varieties
Acyrthosiphon pisum
hazard stress
defensive enzyme
nutrients
