摘要
作者应用外源性DNA转染技术,以人鼻咽癌组DNA,在体外对NIH/3T3细胞进行转染实验。结果观察到在第1、2轮转染中,均获得明显转化灶。并以人Alu顺序为探针,经分子杂交证明1、2轮转化细胞DNA中含有人顺序。以转化细胞注入裸鼠皮下,能诱发形成纤维肉瘤。本实验表明人鼻咽癌DNA中存在具有转化活性的转化基因(癌基因),并已转移至细胞内,发生了明显的恶性转化。作者应用免疫组化技术,观察了Ha-ras基因产物p21蛋白质在人鼻咽癌中的表达。其结果表明,p21在人鼻咽癌中具有阳性表达,其阳性率为90.4％。
Oncogene of nasopharyngeal carcinoma(NPC) by means of external origin DNAtransfection experiment and its gen pro-ducts by immunohistochemical method havebeen studied. These DNAS were isolatedfrom human primary poorly differentiatedNPC tissues and were transfected into NIH/3T3 mouse fibroblasts to induce the foci ofthe morphologically transformed cells in theculture, while DNAs of normal placentatissues failed to do so. The DNAs wereextracted from the primary and secondarytransformed cells to analyse human se-quence with human Alu sequence probe. Thehuman sequence has been detected in theDNAs of the primary and secondary trans-formed foci cells, while none of the humansequence was detected in the DNAs ofthe control. The results indicated thathuman transforming sequences had beenintegrated into transformed cells. The malig-nant properties of the transformed foci cellswere evidenced by tumorigenic expreimentof nude mice. The transformed foci cellswere inoculated subcutaneously in the nudemice and induced fibrosarcoma in vivo.Thetumorigenic rate was 87.5%. It was furtherdemonstrated that DNAs from human NPCpossessed carcinogenicity and induced malig-nant transformation. The primary result revealed that thetransforming gene of NPC may be homolo-gue to Ha-ras oncogene. The expression ofHa-ras gene products-p21 has been studiedin human NPC tissues. The primary resultsshowed a positive expression of p21 in hu-man NPC tissues by immunohistochemicalmethod. The positive rate was 90.4%.
出处
《华西医科大学学报》
CAS
CSCD
1989年第4期347-351,共5页
Journal of West China University of Medical Sciences
基金
四川省卫生厅科学基金
关键词
鼻咽癌
癌基因
转染实验
鼻咽肿瘤
Oncogene
Transfection experiment
Human Alu sequence probe Molecular hybridization
Tumorigenesis of nude mice
Immunohistochemical
Nasopharyngeal carcinoma
