摘要
目的观察丙戊酸钠(VPA)与顺铂(DDP)联合应用对人骨肉瘤细胞株U-2 OS增殖、凋亡的影响,并探讨其可能机制。方法培养U-2 OS细胞,分为A、B、C、D组,A组加入35 mmol/L VPA+20 mg/L DDP,B组加入35 mmol/L VPA,C组加入20 mg/L DDP,D组未加任何药物。CCK-8法检测细胞增殖,流式细胞仪检测细胞周期及凋亡,RT-PCR法检测细胞凋亡相关基因(Caspase-3、Fas)。结果与D组比较,A、B、C组细胞存活率降低,G0/G1期细胞增加,凋亡率及Fas、Caspase-3相对表达量升高;与C组比较,A组细胞存活率降低,G0/G1期细胞增加,凋亡率及Fas、Caspase-3相对表达量升高;P均<0.05。结论 VPA联合DDP可抑制U-2 OS细胞增殖,促进其凋亡,且较单用VPA或DDP作用更为明显;其机制可能与Fas、Caspase-3表达上调有关。
Objective To investigate the effect and mechanism of the sodium valproate （VPA） combined with cispla- tin （DDP） on proliferation and apoptosis of human osteosarcoma cell line U-2 OS. Methods The osteosarcoma U-2 OS cells were cultured in vitro and were divided into groups A, B, C and D. The groups A, B and C were separately treated with 35 mmol/L VPA ＋20 mg/L DDP, 35 mmol/L VPA and 20 mg/L DDP, and the group D was not treated. The prolif- eration was assayed by CCK-8. The cell cycle andapoptosis were analyzed by flow cytometry （FCM）. The gene expression of Fas and Caspase-3 was detected by RT-PCR. Results Compared with group D, the cell viabilities of groups A, B and C were decreased, the cells in the G0/G1 phase：were increased, the apoptosis rate and the expression levels of Fas and Caspase-3 genes were increased ; compared with group C, the cell viability of the group A decreased, the cells in the Go/ G, phase were increased, the apoptosis rate and the expression levels Fas and Caspase-3 genes increased. The difference was statistically significant （ all P 〈 0.05 ）. Conclusions VPA combined with DDP could inhibit the cell proliferation and induce to apoptosis of human osteosarcoma U-2 OS, and the effect is more obvious than that of using only one of them. The mechanism may be closely related to the up-regulated expression of Fas and Caspase-3.
出处
《山东医药》
CAS
北大核心
2016年第3期11-13,共3页
Shandong Medical Journal
关键词
骨肉瘤
丙戊酸钠
顺铂
osteosarcoma
sodium valproate
cisplatin
