摘要
目的:探讨分析格列齐特对2型糖尿病大鼠肾功能、肾脏病理学、缺氧诱导因子-1α(hypoxia Inducible factor-1α,HIF-1α)及血管内皮生长因子(vascular endothelial growth factor,VEGF)影响。方法:48只雄性SD大鼠,随机分为空白组(control group,CG),模型组(model group,MG)以及格列齐特组(gliclazide group,GG),每组16只,MG组和GG组高脂饮食6个月诱导2型糖尿病大鼠模型,造模成功后GG组给予格列齐特24 mg/(kg·d)灌胃治疗,其他二组予生理盐水参照;治疗6周和12周后,每组均处死大鼠各半,收集血清及肾脏行相关检测。结果:CG肾组织切片层次分明,无炎症反应,MG组有明显的炎症反应,治疗6周后明显改善,治疗12周后进一步改善;治疗6周后血清肾功能指标血尿素氮、血肌酐和血尿素GG组明显低于MG组(P<0.05),治疗12周后进一步降低;肾组织HIF-1α及VEGF蛋白和mRNA表达,治疗6周后GG组明显低于MG组(P<0.05),治疗12周后进一步降低。结论:格列齐特可抑制HIF-1α及VEGF的表达,改善2型糖尿病大鼠肾功能。
Objective： To investigate the effects of gliclazide on the kidney function,pathology and hypoxia inducible factor-1α（ HIF-1α） and vascular endothelial growth factor（ VEGF） expression in type 2 diabetic rats induced by high fat diet. Methods： Forty-eight male SD rats were randomly divided into control group（ CG）,model group（ MG） and gliclazide group（ GG）（ n = 16 for each）,Rats in MG and GG groups were given high fat diet for six months to induce type 2 diabetic models. The eligible animals in GG group were intragastrically administered with gliclazide in dose of 24 mg /（ kg·d）,and the other two with saline as placebo. By the 6th week and 12 th week after treatment,half of rats in each group were sacrificed to obtain the serum and kidney tissues for examination. Results： Renal tissue sections in the CG group remained distinct with no inflammatory reaction. Although the renal tissues in MG group demonstrated significant inflammation,yet it was managed and improved after 6 and 12 weeks of treatment,respectively. The index suggestive of renal function,including blood urea nitrogen,serum creatinine and blood urea was significantly reduced in GG group by the 6th week,further decrease occurred by the 12 th week（ P〈0. 05）. Expression of HIF-1α,VEGF protein and mRNA in the renal tissues was significantly lower in GG group than MG group after six week of treatment,and the expression was further down-regulated by the 12 th week（ P〈0. 05）. Conclusion： Gliclazide may inhibit the expression of HIF-1α and VEGF and improve kidney function of type 2 diabetic rats induced by high fat diet.
出处
《皖南医学院学报》
CAS
2016年第1期8-11,共4页
Journal of Wannan Medical College
基金
湖北省自然科学研究基金项目(2013CFC058)
