摘要
目的探讨长链非编码RNA(lncRNA)MEG3对食管癌细胞增殖和侵袭等生物学行为的调控作用。方法收集食管癌手术患者的肿瘤组织42例(肿瘤组)及与其配对的肿瘤旁正常食管组织42例(对照组),通过实时定量聚合酶链反应(RT-PCR)检测MEG3的表达水平并比较其在肿瘤和正常组织中的表达差异;采用聚乙烯亚胺(PEI)和脂质体分别进行MEG3过表达或干扰消除实验;采用噻唑蓝(MTT)、Transwell小室来对各组的KYSE30细胞的增殖活性和侵袭活性进行检测。结果 RT-PCR的结果显示,与正常组织相比,lncRNA MEG3在食管癌组织中呈现低表达。过表达MEG3抑制食管癌细胞系KYSE30细胞增殖,敲除MEG3之后,能够增加食管癌细胞的增殖和侵袭作用。结论 lncRNA MEG3参与食管癌的发生和发展,在食管癌中作为一个肿瘤抑制基因发挥着关键作用。
Objective To investigate the regulating effect of long non-coding RNAs（lncRNAs）MEG3 on the biological behaviors of proliferation and invasion in esophageal cancer cells.Methods 42 samples of tumor tissue（tumor group）in the operative patients with esophageal cancer and 42 samples of paracancerous normal esophageal tissue（control group）were collected and the expression level of MEG3 was detected by using real-time quantitative PCR（RT-PCR）.The expression differences of MEG3 were compared between the tumor tissue and the normal tissue.Polymine（PEI）and liposome were adopted to conduct the over-expression or the interference elimination tests of MEG3;MTT assay and Transwell chamber were performed to detect the proliferation and invasion activities of KYSE30 cell in each group.Results The RT-PCR results revealed that compared with the normal tissue,lncRNA MEG3 showed the low expression in esophageal cancer tissue.Moreover,over-expression of MEG3 inhibited KYSE30cell proliferation,however,after eliminating MEG3,the proliferation and invasion of esophageal cancer cells were increased.Conclusion LncRNA MEG3 participates in the development and progression of esophageal cancer and plays a key role as a tumor inhibiting gene in esophageal cancer.
出处
《检验医学与临床》
CAS
2016年第2期153-156,159,共5页
Laboratory Medicine and Clinic
基金
国家科技基础条件资助项目(2005DKA32403-31)
