摘要
【目的】为快速分析苦楝Melia azedarach不同种源提供方法,揭示苦楝遗传多样性,为苦楝的开发、利用及选择育种提供一定的理论依据。【方法】从783对SRAP引物组合中筛选出20对多态性较高的引物组合,对苦楝国内全分布区的37个种源和肯尼亚1个种源样品进行SRAP遗传多样性分析。【结果】20对引物组合共扩增出242条谱带,其中多态性条带101条,多态性百分率平均值为40.89%,每对引物组合扩增条带5~17条,平均每对引物扩增条带12.1条;其多态性信息量（Polymorphism information content,PIC）值介于0.188~0.488,平均值为0.299。基于UPGMA法聚类以0.350为阈值可将38个苦楝种源划分为7类,在此基础上,去掉2个种源,将其余36个种源划分5个地理类群。【结论】SRAP分子标记可以很好地反映苦楝的遗传多样性,聚类类群划分结果有明显的地理趋势和气候生态特征。
【Objective】To set up a method for rapid detection of different provenances,to investigate the genetic diversity,and to provide a theoretical basis for development,utilization and selective breeding of Melia azedarach.【Method】Twenty SRAP primer combinations（ PCs） with relatively high polymorphisms from 783 PCs were selected to analyze the genetic diversity of 37 provenances from all distribution areas nationwide and 1 provenance from Kenya. 【Result】The results showed 242 clear bands were amplified by20 PCs,among which 101 bands（ 40. 89%） were polymorphic. The number of bands of each PC ranged from 5 to 17,with an average of 12. 1. The polymorphism information content（ PIC） values of these PCs ranged from 0. 188 to 0. 488,with an average of 0. 299. Thirty-eight provenances could be divided into 7categories based on UPGMA（ with a threshold value of 0. 350）,and furthermore,by excluding 2 provenances,the rest 36 provenances could be divided into 5 geographic groups. 【Conclusion】SRAP markers can be used to effectively evaluate the genetic diversity of Melia azedarach,and the results of cluster analysis demonstrate clear geographical trends and climatic-ecological characteristics.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2016年第1期70-74,共5页
Journal of South China Agricultural University
基金
广东省林业科技创新项目(2011KJCX002)
关键词
苦楝
种源
遗传多样性
SRAP
Melia azedarach
provenance
genetic diversity
SRAP
