摘要
目的:探讨利用漆酶破壁结合超声波辅助法提取灵芝孢子粉中灵芝酸(ganoderma acid,GA)组分以及GA对D-半乳糖所致亚急性衰老小鼠的体内抗氧化活性的影响。方法:利用正交试验将工艺条件优化;D-半乳糖连续皮下注射进行亚急性衰老小鼠造模,建模同时,以抗坏血酸为阳性对照组,GA设立高、中、低剂量组对衰老模型小鼠进行灌胃,另设立空白对照组和模型对照组,灌胃0.5%的羧甲基纤维素钠溶液(sodium carboxymethyl cellulose,CMC-Na),每周测一次体质量,6周后分别测所有小鼠血清、肝脏、脑组织的总超氧化物歧化酶(total superoxide dismutase,T-SOD)活力、丙二醛(malondialdehyde,MDA)含量,血清和脑组织的谷胱甘肽过氧化物歧化酶(glutathione peroxidase,GSH-PX)活力、血清和肝脏的总抗氧化能力(total antioxidant capacity,T-AOC)值。结果:得到最优工艺条件为料液比1∶60、加酶量0.04 g/m L、超声3次、每次超声时间3 h,在此工艺条件下得到GA的提取率为1.69%;GA可明显提高小鼠体内T-SOD、GSH-PX活力和T-AOC值,降低MDA含量。结论:得到GA的最佳提取工艺稳定可行;GA能显著提高衰老模型小鼠的体内抗氧化能力。
The extraction of ganoderma acid （GA） from Ganoderma lucidum spore was explored by the combined use of cell wail disruption with laccase and ultrasonic-assisted extraction. Besides, this study also examined the antioxidant effect of GA in subacute senile mice induced by D-galactose. Methods： The extraction process was optimized through orthogonal array experiments. A subacute senile mouse model was established by continuous subcutaneous injection of D-galactose into the nape of the neck. Using ascorbic acid as positive control group, the mice in the high, moderate and low dose groups were given GA by gavage, while those in the blank and model control groups were given 0.5% CMC-Na by gavage. Body weights of these mice were measured once a week for six weeks. After the experimental period, total superoxide dismutase （T-SOD）, the levels of malondialdehyde （MDA） in the serum, liver and brain of mice, the activities of glutathione peroxidase （GSH- Px） in the serum and brain, and total antioxidant capacity （T-AOC） in the serum and liver were determined. Results： The optimal conditions for GA extraction were determined as follows： solid/liquid ratio, 1：60; enzyme concentration, 0.04 g/mL; ultrasonic time, 3 h; and number of ultrasonic treatments, 3. Under these conditions, the maximum yield of GA of 1.69% was obtained. Conclusions： The optimized extraction process is stable and feasible. The GA extracted from Ganoderma lucidum spore could obviously improve T-SOD and GSH-Px activities as well as T-AOC capacity and reduce MDA content in mice. Together, these results implied the obvious anti-aging effects of the GA on subacute senile mice induced by D-gaiactose.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第24期89-94,共6页
Food Science
关键词
漆酶
灵芝酸
D-半乳糖
衰老
抗氧化
laccase
ganoderic acid
D-galactose
aging
antioxidant effect
