摘要
利用仿生硅化技术将苯丙氨酸解氨酶(PAL)和F3O4磁性纳米颗粒共包埋在仿生硅胶中,制备出磁性的仿生硅化的固定化酶。研究固定化条件对PAL固定化的影响及固定化PAL的催化性能。获得的固定化酶优化制备条件:2 m L浓度为0.8 mol/m L正硅酸甲酯水解液,1 m L质量浓度为20 mg/m L磁性纳米颗粒和酶添加量5m L(0.86 U/m L)时,所得固定化酶的最大酶活回收率是52%。与游离酶相比,固定化PAL的温度稳定性、p H和储存稳定性,以及变性剂耐受性都有较大提高,重复使用6次,固定化PAL仍能保持初始酶活的20%。
In this study, the magnetic enzymes-silica composites were prepared by co-entrapping phenylalanine ammonia lyase （PAL） from Rhodotorula gluinis and magnetic nanoparticles in biomimetic silica. The conditions for the preparation of encapsulated PAL were optimized. Moreover, stability of the encapsulated PAL was also examined. The maximum activity recovery （52%） of PAL was achieved when 2 mL tetramethoxysilane （TMOS） of 0.8 tool/L, 1 mL magnetic nanoparticles of 20 mg/mL, and 5 mL PAL （0.86 U/mL） were used. Compared with free PAL, the encapsulated PAL showed excellent properties in pH, thermal and storage stabilities, as well as the tolerance against denaturants. In addition, the encapsulated PAL still retained 20% of its initial activity after consecutive 6 cvcles.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第11期29-33,共5页
Food and Fermentation Industries
基金
国家自然科学基金(21072041)
河北省自然科学基金(B2014208054)
河北省大学生创新创业训练计划项目(201410082024)
关键词
苯丙氨酸解氨酶
仿生硅化
共固定化
固定化酶
磁性纳米颗粒
phenylalanine ammonia lyase
biomimetic silica
co-immobilization
immobilization enzyme
magneticnanoparticles
