摘要
为明确西藏设施辣椒根腐病病原,从西藏采集典型根腐病标本,利用组织分离法分离得到9个真菌分离物,经柯赫氏法则确定2株真菌可引起西藏设施辣椒根腐病,其中2号菌株在分离菌落中出现频率为43.6%,为优势菌株。对其进行孢子形态鉴定、通用ITS序列及镰孢菌专用引物tef1-a和cbh-c序列扩增分析将其鉴定为尖镰孢菌(Fusarium oxysporum)。
In order to explicit the pathogeny of Capsicum root rot in Tibet greenhouse,the typical disease samples were collected from mainly Capsicum cultivated areas in Tibet. Nine strains were isolated from the diseased Capsicum,two of the isolates was tested be the pathogeny of Tibet greenhouse capsicum root rot according to Koch＇s rule. the dominant strain No. 2 had the high frequencies of 43.6 %, which was identified as Fusarium and common Fusarium special primer tell oxysporum by morphology of spore, ITS sequence analysis -a and cbh-c sequence analysis.
出处
《西北农业学报》
CAS
CSCD
北大核心
2015年第12期139-143,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
西藏自治区科技厅重点科研项目(2012)~~
关键词
辣椒
根腐病
病原
鉴定
Capsicum
Root rot i Pathogenl Identification
