摘要
针对离心式微流控生化分析芯片的结构特点,设计了一种基于吸光度检测技术的光探测系统。通过双波长检测方法降低了闪烁氙灯光强输出稳定性差对吸光度检测结果的影响,采用同步触发的定位方式实现了检测过程中芯片的精确定位,并分析了进样误差对吸光度检测结果的影响。实验结果显示,采用双波长检测方法时系统吸光度重复性为0.0994%CV(变异系数);同步触发定位误差极小,在该种定位方式下的吸光度重复性为0.1429%CV;样品与试剂的进样重复性小于2%CV,满足国家标准。该系统结构简单,易于集成,检测结果稳定可靠,在医学临床诊断领域具有重要的实用价值。
Taking advantage of the centrifugal microfluidic chip for biochemical analysis, an optical detection system based on absorbance detection technology is designed. The effect of the poor light output stability of xenon flash lamp on test results is reduced by dual wavelength detection. The chip is located precisely by synchronous trigger positioning in the detection process. The effect of sampling error on the test results is also analyzed. The test results show that the absorbance repeatability of the system is 0.0994% by dual wavelength detection; the locating error is extremely small, it is 0.1429% by synchronous trigger positioning; and the sampling error is lower than 2%, which meets the requirements of national standards. The system has the advantages of simple structure, easy integration, and stable and reliable test results, it is promising in medical diagnosis.
出处
《激光与光电子学进展》
CSCD
北大核心
2015年第12期148-153,共6页
Laser & Optoelectronics Progress
基金
国家863计划(2015AA042402)
国家自然科学基金(51205381)
吉林省双十工程重大科技攻关项目(201402011GX)
吉林省科技发展计划(20140101057JC
20130522185JH)
中国科学院科研装备研制项目(yz201353)
关键词
生物光学
光探测系统
吸光度检测
定位误差
进样误差
微流控芯片
biotechnology
optical detection system
absorbance detection
locating error
sampling error
microfluidic chip
