摘要
建立了有机溶剂萃取、硅胶固相萃取柱净化、液相色谱-串联质谱法（LC-MS/MS法）测定食用油脂中EPA 16种多环芳烃的检测方法。EPA 16种多环芳烃的定量限分别为0.02~0.43μg/kg,回收率为86.5%~104.6%,日内精密度小于6%,日间精密度小于5%。在40个受测油脂样品中,EPA 16种多环芳烃的含量范围为11.68~146.06μg/kg。对照我国GB 2716规定,所有受测样品中苯并（a）芘含量均不超过≤10μg/kg的限量标准。然而,8个油样的苯并（a）芘含量超过了欧盟≤2μg/kg的限量标准,10个油样的PAH4含量超过了欧盟≤10μg/kg的限量标准。
A liquid chromatography tandem mass spectrometry（ LC- MS / MS） method for determination of 16 kinds of Environmental Protection Agency（ EPA） priority PAHs in edible vegetable oils has been studied and established. The sample preparation was conducted with the organic solvent extraction and silica SPE purification procedure and subsequently analyzed by LC- APPI- MS / MS. The new method overcomed the shortcomings of tradition approaches as involved,complex and laborious operations,time and solvent consuming,low sensitivity and selectivity and matrix interference,etc. The quantification limit of 16 kinds of PAHs had a range from 0. 02 ~ 0. 43 μg / kg; the recoveries from 86. 5% ~ 104. 6%; the intra- day and inter- day precision less than 6% and 5% respectively.Within the tested 40 samples,the concentration of 16 kinds of PAHs had a range from 11. 68 μg / kg to 146. 06 μg /kg. The concentration of Bap was lower than the limitation level of GB 2716. However,8 kinds of samples within the total tested vegetable oils had exceeded the maximum limit as 2 μg / kg for Ba P; 10 kinds of samples had exceeded 10μg / kg as standard for PAH4 by EU.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2015年第12期114-119,共6页
Journal of the Chinese Cereals and Oils Association
基金
国家自然科学基金(31271884)
河南省食用油脂倍增计划(豫财贸[2010]169号)
河南省科技攻关项目(152102210262)
