摘要
以一串红35及35-1为试验材料,采用实时荧光定量PCR技术,研究了S-腺苷甲硫氨酸脱羧酶(SAMDC)基因在35及35-1不同发育时期、不同部位的表达情况。结果表明:幼苗期腋芽未形成和腋芽形成后35中SAMDC基因的相对表达量均低于35-1;营养生长期35的茎节、根尖及侧枝顶芽中SAMDC的表达量均高于35-1,且差异显著。试验表明,SAMDC在一串红幼苗顶芽中相对表达量高,可促进植株细胞分裂分化形成侧芽;营养生长期SAMDC相对表达量低,可促进分支和枝条伸长生长。
Taking Salvia splendens 35 and 35-1as materials,using real-time fluorescence quantitative PCR technology to analysis the S-adenosylmethionine decarboxylase（SAMDC）gene relative expression in 35 and 35-1of different parts and developmental stages.The results showed that in seedling stage,before and after axillary buds formed SAMDCrelative expression in 35 was lower than that in 35-1.In vegetative stem,root tips and lateral buds were higher than in 35-1.Difference was significant.It was speculated that SAMDC high expression in seedling bud could promote plant cell division and differentiation to formation lateral bud.In vegetative growth stage,low expression could promote branch and elongation branches growth.This study would help to explore the effect of SAMDCgene in Salvia splendens 35 and 35-1collateral development and bulit a foundation of Salvia splendens morphogenesis studies.
出处
《北方园艺》
CAS
北大核心
2015年第24期90-94,共5页
Northern Horticulture
关键词
一串红
SAMDC
侧枝
实时荧光定量PCR
Salvia splendens
SAMDC
lateral branch
real-time fluorescent quantitative PCR
