摘要
目的研究Roux-en-Y胃转流术(RYGB)对2型糖尿病(T2DM)大鼠血清炎症因子及内脏(附睾)脂肪组织胰岛素受体底物(IRS)-1和IRS-2蛋白表达量的影响,并探讨RYGB治疗T2DM的可能机制。方法将40只SD大鼠建成T2DM模型后,按干预方式将其分为T2MD-RYGB组(n=14)、T2MD-假手术组(n=10)、T2MD组(n=10),另取正常喂养大鼠为对照组(n=10)。T2MD-RYGB组及T2MD-假手术组大鼠分别行RYGB及胃十二指肠离断原位吻合术干预。于术前及术后第1、4、8周(未行手术干预的大鼠与手术大鼠同步检测)检测各组大鼠的空腹血糖(FPG)、空腹血清胰岛素(FINS)、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、游离脂肪酸(FFA)水平及计算稳态模型胰岛素抵抗(HOMA-IR)和脂肪组织胰岛素抵抗(Adipo-IR)指数,术后第8周时获取大鼠附睾脂肪组织,测定其IRS-1、IRS-2蛋白表达量。结果术前,T2MD-RYGB组、T2MD-假手术组、T2MD组糖尿病大鼠FPG、FINS、TNF-α、CRP、FFA水平及HOMA-IR、Adipo-IR指数均明显高于对照组(P<0.05)。术后第4周和第8周时,T2MD-RYGB组以上各项指标(除外第4周时FINS值)与术前比较均有所下降,但高于对照组水平(P<0.05);其余各组术后不同时相与术前相比,差异无统计学意义(P>0.05)。T2MD-RYGB组大鼠附睾脂肪组织中IRS-1和IRS-2蛋白表达量较T2MD-假手术组、T2MD组明显增加(P<0.05),但明显低于对照组(P<0.05)。结论RYGB通过上调脂肪组织中IRS-1和IRS-2蛋白表达水平,使其胰岛素敏感性增强,从而降低血清炎症因子水平,最终改善机体胰岛素抵抗,是该术式治疗T2DM的可能机制。
Objective To study effect of expression levels of serum inflammatory factors and insulin receptor substrate （IRS）-I/2 in visceral adipose tissue after Roux-en-Y gastric bypass （RYGB） on type 2 diabetes mellitus （T2DM） rats, and explore possible mechanism in treatment of T2DM. Methods The T2DM rats models were established, which were divided into 3 groups by intervention： T2MD-RYGB group （n=14）, T2MD-sham operation （T2MD-SO） group （n=10）, and T2MD group （n=10）, and 10 normal rats were selected as control group. The rats of the T2MD-RYGB group were received the RYGB, and of the T2MD-SO group were received transection and reanastomosis of the gastroin- testinal tract. The fasting plasma glucose （FPG）, fasting insulin （FINS）, C-reaction protein （CRP）, tumor necrosis factor-ct （TNF-a）, free fatty acid （FFA）, homestasis model assessment for insulin resistance （HOMA-IR）, adipose tissue insulin resistance （Adipo-IR） were tested respectively before operation and on week 1, 4, 8 after operation （synchronous detec- tion of rats with or without surgical intervention）. The IRS-1 and IRS-2 protein contents of the rat epididymal adipose tissue were tested on week 8 after operation. Results The FPG, FINS, CRP, TNF-a, FFA levels, and HOMA-IR, Adipo-IR indexes in the T2DM rats were significantly higher thart those in the normal rats （P〈0.05） before operation, the above indicators on week 4, 8 after operation were significantly lower than those before operation in the T2MD-RYGB group （P〈0.05）. The differences of changes among the other groups were not statistically significant （P〉0.05）. The IRS-1 and IRS-2 protein expressions in the adipose tissue of the rats were significantly increased in the T2MD-RYGB group as compared with these indicators in the T2MD group and T2MD-SO group （P〈0.05）, but which were significantly lower than those in the control group （P〈0.05）. Conclusions RYGB could increase IRS-1/2 expression levels in adipose tissue, which could enhance insulin sensitivity, decrease serum inflammatory factors levels, and improve insulin resistance ultimately. This might be one of the mechanisms in treatment of T2DM.
出处
《中国普外基础与临床杂志》
CAS
2015年第12期1460-1465,共6页
Chinese Journal of Bases and Clinics In General Surgery
基金
2015年佳木斯大学科学技术校级面上项目(编号:13Z1201540)
黑龙江省自然科学基金面上项目(编号:D201127)
佳木斯大学研究生创新基金项目(编号:LM2015_034)
佳木斯大学研究生创新基金项目(编号:LM2015_030)~~
