摘要
应用细胞杂交瘤技术建立了分泌抗急性非淋巴细胞白血病M_2型单克隆抗体1D_1、1C_2和1D_6细胞株,连续传代11个月,稳定地分泌单克隆抗体。用间接免疫荧光方法检测,这3株细胞分泌的单克隆抗体与急性非淋巴细胞白血病M_2型病人白血病细胞呈阳性反应;与M_1、M_3、M_4、M_5型病人白血病细胞呈阴性反应;与慢性粒细胞白血病、慢粒急变、急性淋巴细胞白血病及正常人白细胞也呈阴性反应,说明单克隆抗体有很好的特异性。
By the application of hybridoma technique we have established 3 cell strains designated as 1 D 1, 1 C 2 and 1 D6, which all the strains secrete anti-acute nonlymphocytic leukemia M2-type monoclonal antibodies individually. These strains secrete the McAb steadily after passed on propagation over 11 months. The McAb secreted by these cell strains all give positive reactions with the leukemia cells from actue non-lymphocytic leukemia M2-type pationt, but not with cells from M1, M3, M4, and M5 type patients. These McAb also can not react with cells from chronic myelocytic leukemia and its acute transformation phase and acute lymphocytic leukemia patient. The McAb can not react with white cells from normal adults too. From the above metioned we may conclude that the McAb secreted by these cells possess a remarkable specificity. Therefore it is applicable for the immuno-typing diagnosis of acute non-lymphoc yt ic leukemia and researches on target directed therapeutics.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1991年第2期87-91,共5页
Chinese Journal of Immunology
关键词
白血病
单克隆抗体
杂交瘤
Acute non-lymphocytic leukemia Mi-type
Monoclonal antibody hybridoma
