摘要
本文介绍了免疫细胞ACTH受体(ACTHR)测定法并首次报道了大鼠胸腺细胞具有高亲和力和低亲和力两类ACTHR。方法学研究表明,胸腺细胞数量以1～2×10~6个/管、反应时间20分钟较佳;竞争结合实验结果表明,非标记ACTH的IC_(50)值为800nM;当加入的^(125)I-ACTH为2.5 nM时,胸腺细胞ACTHR已基本饱和,根据饱和曲线进行Scatchard作图分析表明,大鼠胸腺细胞具有两类亲和力不同的ACTHR。应激可致胸腺细胞ACTHR急骤减少。
In this paper, it was reported that rat thymocytes harbor both high and low affinity ACTHR. The proper number of thymocytes used for deterting ACTHR varies from 1×10~6 /0.2 ml to 2 ×10~6 /ml, the maximum binding of ACTH to ACTHR occurs at 20 minutes. The IC_(50) for ACTH concentration is 800 nM or so. The saturation curves show that thymocyte ACTHRs are saturated at the concentration of 2.5 nM of ^(125)IACTH. There are high affinity ACTHR of 840 sites/cell on thymocyte (KD=0.15nM, Bmax = 2. 8 fmol/2 × 10~6 cells); whereas low affinity ACTHRs are 12400 sites/cell (KD=4.2 nM, Bmax = 41.5 fmol/2 × 10~6 cells).Moreover amputation stress can reduce thymocyte ACTHR remarkably.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1991年第1期14-16,共3页
Chinese Journal of Immunology
关键词
免疫细胞
ACTH
受体
胸腺细胞
thymocyte ACTHR
IC_(50)
saturation curves
scatchard plot
stress
