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纺锤体观察仪在卵胞浆内单精子显微注射技术中的应用 被引量：1

APPLICATION OF POLSCOPE IN INTRACYTOPLASMIC SPERM INJECTION
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摘要 目的：探讨纺锤体观察仪在卵胞浆内单精子显微注射技术（ICSI）中的应用价值。方法：2011年1~12月因男方因素行ICSI助孕的患者共216例,以ICSI操作中是否使用纺锤体观察仪分为实验组54例,对照组162例,比较两组的正常受精率、卵裂率、优质胚胎率、多精受精率、妊娠率以及种植率。结果：两组正常受精率、卵裂率、优胚率、种植率、临床妊娠率比较差异无统计学意义（P〉0.05）,实验组多精受精率显著低于对照组（P〈0.05）。结论：将纺锤体观察仪应用于ICSI可以避免损伤纺锤体,使ICSI更加安全可靠,可以降低多精受精的发生率,但不能改善其妊娠结局。 Objective：To observe the application of polscope in intracytoplasmic sperm injection（ICSI）.Methods：We studied 216 cycles of intracytoplasmic sperm injection and embryo transfer in patients with male factor from January,2011 to December,2011.Among 216 cycles,54cycles were with polscope in intracytoplasmic sperm injection（experimental group）,and 162 were without polscope（control group）.The normal fertilization rate,cleavage rate,good-quality embryo rate,pregnancy rate and implantation rate were compared.Results：No significant difference was found in two groups（P〉0.05）in normal fertilization rate,cleavage rate,rate of high quality embryos,pregnancy rate and implantation rate.Conclusion：Using the polscope to observe the spindle in intracytoplasmic sperm injection may avoid injury,and result in not only safer and more reliable ICSI but also a lower incidence of polypronuclear,although it cannot improve the pregnancy outcome.
作者 孙燕萍 牛向丽 方伟芬 陈自洪 公方强 黄华 乃东红 施丽
机构地区 广西壮族自治区人口和计划生育研究中心生殖医学科
出处 《广西医科大学学报》 CAS 2015年第4期582-584,共3页 Journal of Guangxi Medical University
基金 广西壮族自治区人口和计划生育委员会科研资助项目(No.桂人口计生研1001 No.桂人口计生研1305 No.桂人口计生研1101)
关键词 纺锤体观察仪 卵胞浆内单精子显微注射技术 纺锤体 polscope inntracytoplasmic sperm injection spindle
分类号 R713 [医药卫生—妇产科学]
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参考文献9

  • 1Silva CP, Kommineni K, Oldenbourg R, et al. The first polar body does not predict accurately the location of the metaphase]I meiotic spindle in mammalian oocytes [J]. Fertil Steril,1999,71(4) :719-721.
  • 2Hardarson T, Lundin K, Hamberger L. The position of the metaphase II spindle cannot be predicted by the lo- cation of the first polar body in the human oocyteJ]. Hum Reprod ,2000,15(6) : 1372-1376.
  • 3朱亮,孔令红,李红,陈士岭,邢福祺.第一极体不能精确定位人卵母细胞纺锤体[J].第一军医大学学报,2004,24(7):809-811. 被引量：5
  • 4Wang WH, Meng L, Hackett RJ, et al. Rigorous ther mal control during intracytoplasmic sperm injection sta- bilizes the meiotic spindle and improves fertilization and pregnancy rates[J]. Fertil Steril, 2002, 77 (6): 1274-1277.
  • 5Rienzi L, Ubaldi F, Martinez F, et al. Relationship be- tween meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI[J]. Hum Reprod, 2003,18(6) : 1289-1293.
  • 6Dominko T,Chan A, Simerly C, et al. Dynamic imaging of the metaphase II spindle and maternal chromosome- sin bovine oocytes:implications for enucleation efficiency verification, avoidanceof parthenogenesis, and sue- cessful embryogenesis [J]. Biol Reprod, 2000, 62 (1): 150-154.
  • 7Wang WH, Meng L, Hackett RJ, et al. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination [J]. Hum Reprod,2001,16(7) :1464-1468.
  • 8Wang WH, Keefe DL. Spindle observation in living mammalian oocytes with the polarization microscope and its practical use[J]. Cloning Stem Cells, 2002, 4 (3) :269-276.
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二级参考文献14

  • 1Hewitson L, Dominko T, Takahashy D, et al. Unique checkpoints during the first cell cycle of fertilisation after intracytoplasmic sperm injection in rhesus monkeys[J]. Nature Med, 1999, 5(4):431-3.
  • 2Silva CP, Kommineni K, Oldenbourg R, et al. The first polar body does not predict accurately the location of the metaphase Ⅱ meiotic spindle in mammalian oocytes[J ]. Fertil Steril, 1999, 71(4): 719-21.
  • 3Hardarson Th, Lundin K, Hamberger L. The position of the metaphase Ⅱ spindle cannot be predicted by the location of the first polar body in the human oocyte[J]. Hum Reprod, 2000, 15(6): 1372-6.
  • 4Liu L, Trimarchi JR, Oldenbourg R, et al. Increased birefringence in the meiotic spindle provides a new marker for the onset of activation in living oocytes[ J]. Biol Reprod, 2000, 63(1): 251-8.
  • 5Wang WH, Meng L, Hackett RJ, et al. Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy[J]. Hum Reprod, 2001, 16(11):2374-8.
  • 6Maria TZ, Ryszard B, Robert F, et al. Effects offchilling to 0 ℃ on the morphology of meiotic spindles in human metaphase Ⅱ oocytes[J]. Fertil Steril, 2001, 75(4): 769-77.
  • 7Boiso I, Marti M, Santalo J, et al. A confocal microscopy analysis of the spindle and chromosome configurations of human oocytes cryopreserved at the germinal vesicle and metaphase Ⅱ stage [J]. Hum Reprod, 2002, 17(7): 1885-91.
  • 8Oldenbourg R. Polarized light microscopy of spindles [J ]. Methods Cell Biol, 1999, 61: 175-6.
  • 9Wang WH, Meng L, Hackett RJ, et al. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination[J]. Hum Reprod, 2001, 16(7): 1464-8.
  • 10Wang WH, Meng L, Hackett RJ, et al. Rigorous thermal control during intracytoplasmic sperm injectionstabilizes the meiotic spindle and improves fertilization and pregnancy rates [J]. Fertil Steril,2002, 77(6): 1274-7.

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广西医科大学学报
2015年 第4期

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