摘要
为了研究黑蜂王台病毒在中国的发展情况，在吉林省内疑似蜜蜂黑蜂王台病痛料中分离到1株BQCV毒株，记为中国BQCV—JL1（KP119603），对该分离株提取RNA，采用RT—PCR技术扩增其VP基因。结果表明：VP基因测序结果与GenBank中报道的BQCVVP基因序列同源性在89％～99％之间。将VP基因序列与GenBank中报道的17个序列进行系统遗传进化树分析，其中与13本分离株4（AB605360）、13本分离株3（AB605359）、13本分离株2（AB605358）及日本分离株Ac1（AB638414）、日本分离株Am3（AB638413）、日本分离株Am2（AB638412）株同源性最高（99％），而与南非分离株（AF183905）同源性最低（89％）。
To study the development of the Black queen cell virus （BQCV） in China, a strain of BQCV was isolated from a pathological material with suspected BQCV from Jilin province, and named China BQCV - JL1 （ KP119603 ）. The isolate was used to extract its RNA, and then a RT - PCR technique was used to amplify its VP gene. The results showed that the sequencing result of the VP gene shared 95% to 99% homology with the sequences of the VP gene of BQCV reported in GenBank. A systematic phylogenetic tree was used to analyze the gene sequence of the YP gene with 17 sequences reported in GenBank. It was found that China BQCV - JL1 shared highest homology （99%） with the Japanese strain 4（ AB605360）, Japanese strain 3 （ AB605359 ）, Japanese strain 2 （ AB605358 ）, Japanese strain Acl （ AB638414 ）, Japanese strain Am3 （AB638413）, Japanese strain Am2 （AB638412）and shared lowest homology （89%） with the South Africa strain （AF183905）.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2015年第11期48-51,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31001065)
质检总局科技项目(2013IK033)
关键词
蜜蜂黑蜂王台毒
分离株
VP基因
同源性
遗传进化树
Black queen cell virus in honey bees
isolate
VP gene
homology
phyiogenetic tree
