摘要
为研究IGF-Ⅰ对宫内发育迟缓(IUGR)猪胸腺T淋巴细胞分化的促进作用及Notch通路的调控作用,本实验在新生IUGR仔猪胸腺细胞培养体系中添加浓度为0、10、50、100 ng/mL的IGF-Ⅰ,以完全培养基培养的正常仔猪和IUGR仔猪胸腺T细胞分别作为正、负对照组。结果表明:培养第1天,IGF-Ⅰ处理组CD4^+和CD8^+T细胞比例均比正、负对照组高(P<0.05);培养第3天,IGF-Ⅰ10 ng/mL组CD4^+T细胞比例最高,且正对照组显著高于负对照组(P<0.05),但对于CD8^+T细胞来说,IGF-Ⅰ100 ng/mL组最高;培养第5天,正对照组CD4^+、CD8^+T细胞比例都最高,但50 ng/mL IGF-Ⅰ组显著高于负对照组(P<0.05)。在培养全期,IGF-Ⅰ处理组Delta-like1 mRNA表达量均显著高于负对照组(P<0.05),在第1天,IGF-Ⅰ10、50 ng/mL组Delta-like1 mRNA表达量低于正对照组(P<0.05),然而,第3、5天则高于正对照组(P<0.05)。Delta-like4和Notch2 mRNA的表达趋势与Delta-like1 mRNA的相似。本试验结果证明,IGF-Ⅰ能不同程度地改善IUGR猪T细胞的发育,促进CD4^+和CD8^+T细胞的转化与增殖,最佳剂量为IGF-Ⅰ10 ng/mL,培养时间不能超过3 d。IGF-Ⅰ可通过调控Delta-like4、Delta-like1和Notch2的基因表达来双阳性T细胞分化为CD4^+和CD8^+T细胞。
This experiment was conducted to investigate the effect of insulin-like growth factor-I （IGF-I） on T cells development of neonatal intrauterine growth retarded （IUGR） piglet and regulation ofthe Notch pathway. T cells of thymus of neonatal IUGR piglets were cultured with RPMI1640 media containing 0, 10, 50 and 100 ng/mL of IGF-I, with T ceils of normal and IUGR piglets in RPMI1640 media as positive and negative control. The results showed that at d 1, the number of CD4^＋ and CD8^＋ T cells in all group with IGF-I were higher than those in positive and negative control （P〈0.05）. At d 3, the number of CD4^＋ T cellsin IGF-I 10 ng/mL group was the highest, and positive control was higher than negative one （P〈0.05）. At d 5, the number of CD4^＋ and CD8^＋ T cells in positive control werethe highest, and IGF-I 50 ng/mL group was higher than negative control, but lower than positive control.During cell culture, the mRNA expression of Delta-likel in all groups was higher than that in negative control （P〈0.05）. At d l, the mRNA expression of Delta-likel in IGF-I 10 and 50 ng/mL group lower than that in positive control, however, as for d 3 and 5, it was lower （P〈0.05）. The mRNA expression trend of Delta-liked and Notch2 was similar with Delta-likel.Resuhs suggested that, IGF-I couldimprove T-cell subsets development of neonatal IUGR piglets, stimulate CD4^＋ and CD8^＋ T cell proliferation, and the optimal dose of IGF-I is 10 ng/mL and culture time was shorter than 3 d. Maybe IGF-I can stimulate DP T cellsto differentiate into CD4^＋ and CD8^＋ through regulation of Delta-like4, Delta- like1 and Notch2 gene mRNA expression.
出处
《中国畜牧杂志》
CAS
北大核心
2015年第21期29-32,共4页
Chinese Journal of Animal Science
基金
国家自然科学基金(31172216)
