摘要
目的探讨组蛋白去乙酰化酶1(HDAC1)、转录因子E2F1在人肾透明细胞癌中的表达和意义。方法应用免疫组织化学法检测51例肾透明细胞癌组织、24例癌旁正常组织中HDAC1、E2F1的蛋白表达情况。RT-PCR方法检测31例肾透明细胞癌组织、14例癌旁正常组织中HDAC1、E2F1的mRNA相对表达情况。观察HDAC1及E2F1的表达与临床病理特征的关系。结果免疫组化结果显示:HDAC1蛋白在肾透明细胞癌组织中的阳性表达率,高于癌旁正常组织(P<0.05);E2F1蛋白在肾透明细胞癌组织中的阳性表达率,高于癌旁正常组织(P<0.05);HDAC1与E2F1的蛋白表达与患者性别、年龄及瘤体大小无关(P>0.05),而与组织学分级及临床分期有关(P<0.05)。RT-PCR结果显示:HDAC1mRNA在肾透明细胞癌组织中的相对表达量,高于癌旁正常组织(P<0.05);E2F1mRNA在肾透明细胞癌组织中的相对表达量,高于癌旁正常组织(P<0.05);HDAC1与E2F1的mRNA相对表达量与患者性别、年龄及瘤体大小无关(P>0.05),而与组织学分级及临床分期有关(P<0.05)。结论 HDAC1与E2F1在肾透明细胞癌中的表达高于癌旁正常组织,且与组织学分级及临床分期有关,并随组织恶性程度的增加其表达水平逐渐增加,提示与肾透明细胞癌的进展有密切关系。
Objective To investigate the expression and significance of HDAC1 and E2F1 in renal clear cell carcinoma（ RCCC）. Methods Immunohistochemistry was used to detect the expression levels of HDAC1 protein and E2F1 protein in 51 cases of RCCC tissues and 24 cases of peritumoral normal tissues. RT-PCR was used to detect the relative expressions of HDAC1 and E2F1 mRNA in 31 cases of RCCC tissues and 14 cases of peritumoral normal tissues. Moreover the correlation between the expressions of HDAC1,E2F1 and clinicopathological characteristics of RCCC was analyzed. Results Immunohistochemical staining results showed that positive expression rate of HDAC1 protein in RCCC tissues was significantly higher than that in peritumoral normal tissues（ 62. 7% vs 25%,P〈0. 05）. The positive expression rate of E2F1 protein in RCCC tissues was significantly higher than that in peritumoral normal tissues（ 70. 6% vs 37. 5%,P〈0. 05）. The expressions of HDAC1 and E2F1 protein were not correlated to patient＇s sex,age and tumor sizes（ P〉0. 05）,however,which were related with histological grade and clinical stage（ P〈0. 05）. RT-PCR results demonstrated that the relative expression levels of HDAC1 mRNA in RCCC tissues were significantly higher than those in peritumoral normal tissues（ 0. 351 ±0. 151 vs 0. 102 ± 0. 044,P〈0. 05）,furthermore,the expression levels of E2F1 mRNA in RCCC tissues were significantly higher those in peritumoral normal tissues（ 0. 318 ± 0. 092 vs 0. 135 ± 0. 032,P〈0. 05）. The relative expression levels of HDAC1 and E2F1 mRNA were not correlated to patient＇s sex,age and tumor sizes（ P〉0. 05）,however,which were related with histological grade and clinical stage（ P〈0. 05）. Conclusion The expression levels of HDAC1 and E2F1 in RCCC are obviously higher than those in peritumoral normal tissues,which are correlated to histological grade and clinical stage,moreover,which are increased with the increase of malignant degree of tumor,which may be closely related with the progress of RCCC.
出处
《河北医药》
CAS
2015年第19期2885-2888,共4页
Hebei Medical Journal
