摘要
目的:观察调理脾胃针法对糖尿病肾病(diabetic nephropathy,DN)患者氧化应激水平的影响,探讨针刺治疗DN的可能作用机制。方法:将144例DN患者按随机数字表法分为观察组和对照组各72例。在常规糖尿病治疗的基础上,观察组采用调理脾胃针法,取中脘、曲池、合谷、血海、足三里、阴陵泉等穴,对照组取肾俞、太溪、三阴交、阳陵泉、悬钟、关元、手三里等穴,均每日治疗2次,6d为1个疗程,共治疗6个疗程。治疗前及第3和第6个疗程结束后对两组患者血浆丙二醛(malondialdehyde,MDA)、羰基(carbonyl group,PCO)、8-羟基脱氧鸟苷(8-hydroxyguanine,8-OHdG)含量,超氧化物歧化酶(superoxide dismutase,SOD)活性及临床疗效进行评定。结果:治疗3个疗程结束时,两组血浆MDA、PCO、8-OHdG较治疗前升高(P<0.05),SOD活性较治疗前下降(P<0.05)。治疗6个疗程后,两组血浆MDA、PCO、8-OHdG较治疗前明显下降(P<0.01,P<0.05),观察组SOD活性升高(P<0.01),对照组SOD明显下降(P<0.05),观察组较对照组改善明显(P<0.05)。观察组3个疗程后总有效率为50.00%(35/70),对照组为44.44%(32/72),差异无统计学意义(P>0.05)。6个疗程后,观察组总有效率为84.29%(59/70),对照组为55.56%(40/72),观察组优于对照组(P<0.01)。结论:针刺能够改善DN患者抗氧化应激反应的能力,从而改善肾功能,延缓肾损害。而随着治疗时间的延长,调理脾胃针法效果明显优于常规针法。
Objective To observe the effect of＂spleen-stomach needling＂on changes of plasma malondialdehyde（MDA）,protein carbonyl（PCO,an oxidative stress damage biomaker）,8-hydroxyguanine（8-OHdG,an indicator of oxidative DNA damage）and the activity of superoxide dismutase（SOD）levels in patients with diabetic nephropathy,so as to explore its mechanisms underlying improving diabetic nephropathy.Methods Diabetic nephropathy patients were randomized into routine acupuncture（control）group（n=72）and spleen-stomach needling（observation）group（n=70）according to a random digits table and by using multi-centers,randomized and blind methods in the present study.All the patients were received routine medication.In addition,patients of the control group were treated by acupuncture stimulation of Shenshu（BL 23）,Taixi（KI 3）,Sanyinjiao（SP 6）,Yanglingquan（GB 34）,Xuanzhong（GB 39）,Guanyuan（CV 4）,Shousanli（Ll 10）,Waiguan（TE 5）,Yangxi（LI5）,Liangqiu（ST 34）,Shangjuxu（ST 37）,Neiting（ST 44）,Huaroumen（ST 24）,and Dachangshu（BL 25）,and those of the observation group treated by acupuncture stimulation of Zhongwan（CV 12）,Quchi（LI 11）,Hegu（LI 4）,Xuehai（SP 10）,Zusanli（ST 36）,Yinlingquan（SP 9）,Fenglong（ST 40）,Diji（SP 8）,Sanyinjiao（SP 6）,Taichong（LR 3）,Gaohuang（BL 43）,Shenshu（BL 23）,Baihuanshu（BL 30）and Zhongji（CV 3）with spleen-stomach needling method.The treatment of both groups was conducted twice a day for six weeks except Sundays.Plasma MDA,PCO and 8-OHdG contents were detected by using ELISA,and SOD detected using xanthine oxidase method.Results After 3weeks＇ treatment,plasma MDA,PCO and 8-OHdG contents were significantly increased in both control and observation groups,and plasma SOD was obviously reduced in both groups（P〈0.05）.No significant differences were found between the two groups in the plasma MDA,PCO and 8-OHdG contents（P〉0.05）.While after 6weeks＇ treatment,plasma MDA,PCO and 8-OHdG contents were significantly decreased（turning to the normal levels）and plasma SOD level in the observation group was significantly up-regulated（P〈0.01）.Following 3weeks＇ treatment,of the 72 and 70patients in the control and observation groups,10 and 20cases experienced marked improvement,22 and 15were improved,and 40 and 35invalid,respectively.After 6weeks＇ treatment,of the 72 and 70patients in the control and observation groups,12 and 39experienced marked improvement,28 and 20cases were improved,32 and 11invalid,respectively.The therapeutic effect of the observation group was obviously superior to that of the control group（P〈0.01）.ConclusionAcupuncture treatment with spleen-stomach regulation needling can down-regulate plasma MDA,PCO,8-OHdG contents and upregulate SOD activity in diabetic nephropathy patients,which may contribute to its effect in improving the patients＇ symptoms probably by resisting the oxidative stress damage.
出处
《针刺研究》
CAS
CSCD
北大核心
2015年第5期409-414,共6页
Acupuncture Research
基金
国家自然科学基金项目(No.81373784)
关键词
糖尿病肾病
针刺疗法
氧化应激损伤
调理脾胃针法
Diabetic nephropathy
Acupuncture therapy
Oxidative stress injury
Spleen-stomach regulation needling
