摘要
目的：建立简单、快速、灵敏的测定花生壳中木犀草素含量的的液相色谱-单级质谱联用（LC-MS）方法。方法：采用Kromasil C18（4.6mm×50mm,5μm）色谱柱;流动相：甲醇-0.2%甲酸（体积比50∶50）;流速为0.35m L·min-1;柱温为室温;质谱条件：电喷雾离子源（ESI）,选择性离子检测（SIM）,正离子模式。结果：木犀草素在50~2000ng·m L-1浓度范围内,线性关系良好（r2=0.9958）;方法的精密度、重现性和稳定性良好;平均回收率为97.70%。采用该方法测定的花生壳中木犀草素的平均含量为0.21%。结论：该法专属性强、快速、灵敏,可用于花生壳中木犀草素的含量测定。
Objective： To develop a simple, quick, sensitive LC-MS method for the determination of luteolin in peanut shell. Method： The analysis was performed on a Kromasil C18 column （4.6mm×50mm, 5μm） with the mo- bile phase of methanol- water （containing 0.2% formic acid）（v/v=50：50） at a flow rate of 0.35 mL.min-1. The temperature of column was room temperature. The LC-MS system was operated by using an electrospray ionization （ESI） probe in the positive ion mode. Scan mode was in selected ion monitoring （SIM）. Result： The calibration curve of luteolin was linear in the range of 50-2000ng.mL-1 （r2=0.9958）. The precision and repeatability and sta- bility were in line with the sample measurement requirements. The mean recoveries were 97.70%. The average content of luteolin in peanut shell was 0.21%. Conclusion： The method was proved to be selective, quick and sen- sitive and have been successfully applied for determination of luteolin in peanut shell.
出处
《化学工程师》
CAS
2015年第10期26-28,共3页
Chemical Engineer
