摘要
[目的]研究雌激素受体2（ERβ）与肝细胞肝癌（HCC）术后复发的相关性以及其潜在分子机制。[方法]通过q RT-PCR技术与Western Blot检测ERβ在HCC术后两年内复发的8例患者以及10例未复发患者癌组织样本内的表达差异。同时,利用三种交叉验证实验（留一交叉验证、十折交叉验证和蒙特卡罗交叉验证）分析ERβ表达对HCC样本的术后复发预后的分型准确性。此外,进一步预测ERβ下游调控基因,分析下游调控基因显著参与的生物学功能以及信号通路,探究其可能的分子机制。[结果]ERβ基因在术后复发的HCC样本中显著高表达,与术后复发明显相关,且通过交叉验证实验结果发现ERβ对HCC样本有较好的分型准确性,三种验证方法的分型准确率分别为75%（73%～76%）、73%（72%～73%）与71%（71%～72%）。最后通过对ERβ下游调控基因的功能富集分析发现,ERβ显著参与到细胞增殖、RNA转录等HCC术后发展相关的功能与通路中。[结论]ERβ具有良好的HCC术后复发预测效能,具有成为HCC术后复发分子标志物的潜力,具有一定的研究价值。
[Purpose] To investigate the correlation and mechanism between estrogen receptor 2 （ERβ） expression and postoperative recurrence of hepatocellular carcinoma（HCC）. [Methods] The expression of ERβ between 8 patients with postoperative recurrence of HCC and 10 patients with no recurrence of HCC was detected by qRT-PCR and Western Blot. The classified power of ERβ was analyzed by three cross-validation algorithms[leave-one-out cross validation （LOOCV）, 10-fold cross validation （10-fold CV） and Montecarlo cross validation （MCCV）]. Moreover, the downstream regulated genes of ERβ were predicted. Then,biological functional enrichment analysis was used to detect the functions and processes related to these regulated genes. [Results] The expression of ERβ gene was higher in patients with postoperative recurrence of HCC. ERβ had a high predic- tion accuracy for classified HCC [LOOCV：75% （73%～76%）, 10-fold CV ：73% （72%～73%）, MC- CV：71% （71%～72%）]. At last,functional enrichment analysis showed that the regulated genes of ER～ were significantly involving in functions of cell proliferation, apoptosis, differentiation, anglo- genesis and HCC progression related functions and pathways. [Conclusion] ER～ shows a good classified power for recurrence of HCC,and those may be candidate biomarkers of postoperative recurrence of HCC and worth researching.
出处
《肿瘤学杂志》
CAS
2015年第10期835-838,共4页
Journal of Chinese Oncology
关键词
ERΒ
肝肿瘤
术后复发
分子标志物
ERβ
liver neoplasms
postoperative recurrence
biomarker
