摘要
目的 :构建含新克隆的启动子 (PLUNC -p)与增强型绿色荧光蛋白 (EGFP)的表达载体 ,研究该启动子的组织调控特性。方法 :爪蟾转基因系统。结果 :荧光显微镜下观察 ,与广泛性调控子巨细胞病毒 (cy tomegalovirus,CMV)启动子相比 ,PLUNC -p较为特异性地调控绿色荧光蛋白表达于爪蟾幼体的筛弓区及部分表皮。结论 :PLUNC -p很有可能具有上皮组织特异性调控特性 ,这一特性为实现转基因定向表达于上皮组织提供了可能。
AIM: Our study focused on investigation of tissue specific regulatory activity of the newly cloned promoter: PLUNC-p with driving enhanced green fluorescent protein ( EGFP ). METHODS: Transgenic Xenopus Laevis system was applied.RESULTS: The green fluorescence protein directed under PLUNC-p was expressed strictly in branchial arches and epidermis of Xenopus Laevis embryos while CMV promoter showed ubiquitous regulation characteristic.CONCLUSION: PLUNC-p is able to direct epithelia specific expression of EGFP . This property of PLUNC-p might raise the possibility that lead target genes to express tissue-specifically.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第8期881-883,I001,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金重点资助项目 (No.39730 2 0 0 )
国家自然科学基金资助项目 (No .39970 367)
