摘要
目的探讨EB病毒感染与系统性红斑狼疮发生、发展的关系。方法用流式细胞仪双色解析法检测SLE患者外周血CD19+、CD23+B淋巴细胞中EB病毒潜伏膜蛋白LMP1和复制期蛋白ZEBRA的表达。结果活动期患者CD19+细胞和CD23+细胞LMP1的表达率均高于稳定期患者,两组患者均高于对照组,且患者CD23+细胞LMP1的表达率高于CD19+细胞,而对照组两者差异无显著性;ZE-BRA的表达仅见于SLE患者外周血B淋巴细胞中,活动期患者CD19+、CD23+细胞ZEBRA的表达率均高于稳定期患者,且在活动期患者中,CD23+细胞ZEBRA的表达率高于CD19+细胞,而在稳定期患者中,CD23+细胞和CD19+细胞ZEBRA的表达率差异无显著性。结论EB病毒的感染和复制与B淋巴细胞的活化及SLE的发生和发展有关。
Objective To investigate the correlation betw een Epstein-Barr virus(EBV)infection and the initiation and development of syste mic lupus erythematosus.Methods The expression of EBV-LMP1and E-BV-ZEBRAwas detected by two-colour cytofluorography in CD19 + ?CD23 + B lymphocytes in peripheral blood of SLE patients.Results The expression of EBV-LMP1in CD19 + Bcells and CD23+ Bcells was significantly increased in active stage patients compared with that in remission stage patients,an d in patient group compared with that in controls.The frequencies of expression of EBV-LMP1were significantly higher in CD23 + B cells than that in CD19 + Bcells in SLEpatients,but not in normal controls.The expression of EBV-ZEBRAcould be detected in SLE patien ts only,and its expression in CD19 + B cells and CD23 + B cells was significantly higher in a ctive stage than that in remission stage.The frequencies of expression of EBV-ZEBRAwere significantly higher in CD23 + Bcells than that in CD19 + B cells in active stage,but not in rem ission stage.Conclusion EBV infection and replication lead t o overactivation of B lymphocytes,which might be related to the initiatio n and development of SLE.[
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2002年第4期269-271,共3页
Chinese Journal of Dermatology
