摘要
目的探讨泌尿生殖道感染患者中解脲脲支原体（Uu）和沙眼衣原体（Ct）的感染情况,为临床早期诊断及时治疗提供依据。方法应用实时荧光定量PCR（FQ-PCR）技术,对2004年1月-2013年12月门诊皮肤性病科就诊的2 625例不同年龄段的疑似感染患者进行Uu及Ct核酸检测与分析。结果 2 625例患者标本中病原体检测阳性1 138例,阳性率为43.35%,其中,检测Uu阳性815例阳性率31.05%、Ct阳性226例阳性率8.61%、Uu＋Ct阳性97例阳性率3.70%;女性患者送检标本中Uu检测阳性率为44.14%,明显高于男性患者的28.02%;而女性患者Ct阳性率4.86%低于男性患者的9.48%,差异有统计学意义（P〈0.01）;21～30岁和31～40岁年龄患者分别占42.93%和39.92%,其次为41～50岁者,≥60岁患者仅占0.50%,差异有统计学意义（P〈0.01）;2004-2013年Uu检测阳性率呈波浪式上升趋势,而Ct、Uu＋Ct检测阳性率在2004-2011年的波浪式上升后,近两年呈明显下降趋势,差异有统计学意义（P〈0.05）。结论本地区在泌尿系统感染的疑似患者中,主要以Uu、Ct的单独感染为主,以Uu感染占多数,主要集中在青壮年人群中,且这两种病原体感染近十年来有上升的迹象,因此对疑似病例应用实时荧光PCR快速诊断具有重要的临床指导意义。
OBJECTIVE To investigate the distribution of Ureaplasma urealyticum （Uu） and Chlamydia trachoma‐tis （Ct） causing urethritis so as to provide references for early diagnosis and treatments .METHODS Totally 2625 suspected cases received from Jan .2004 to Dec .2013 were detected by real time fluorescence PCR （FQ‐PCR） . RESULTS In 2625 clinical samples ,1138 proved to be positive in pathogen testing and the general infection rate was 43 .35% .Among them ,815 were Uu positive with the rate of 31 .05% and 226 cases were Ct positive with the rate of 8 .16% .Totally 97 cases were proved to be both Uu and Ct positive and the rate was 3 .70% .The in‐fection rate of Uu in females （44 .14% ） was higher than that in males （28 .02% ） （P〈0 .01）;however ,the infec‐tion rate of Ct in females （4 .86% ） was lower than that in males （9 .48% ） .The differences were significant （P〈0 .01） .The results showed that 42 .93% of the patients were between 21 and 30 and 39 .92% were between 31 and 40 .Patients over 60 only accounted for 0 .50% and the differences were significant （P〈0 .01） .Uu positive rate showed a wave‐like increasing trend from 2004 to 2013 ,while Ct or Uu＋Ct positive rate increased from 2004 to 2013 and showed an obvious decreasing trend in recent 2 years .The differences were significant （P〈 0 .05） . CONCLUSION In recent years ,urethritis infections were mainly caused by Uu or Ct and Uu was the main patho‐gen .Young adults are the most susceptible population .In addition ,infections caused by these two kinds of patho‐gens showed an increasing trend;hence FQ‐PCR is important to guide clinical treatment for suspected cases .
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第20期4642-4644,共3页
Chinese Journal of Nosocomiology
基金
贵州省细胞工程中心建设和贵州省省长资金临床应用专项基金资助项目[黔省专合字(2012)107]
