摘要
目的：研究胶质瘤相关癌基因同源蛋白2（Gli2）在骨巨细胞瘤组织中的表达，分析其表达与影像学级别、病理类型、复发的关系，探讨其对骨巨细胞瘤发病及预后的影响。方法收集北京大学人民医院骨与软组织肿瘤治疗中心自2009年1月至2012年12月收治的有完整病历及随访资料的初治骨巨细胞瘤46例，其中良性40例（含侵袭性病例10例），恶性6例。通过免疫组织化学、实时PCR方法检测Gli2在不同类型骨巨细胞瘤中的表达情况，并结合病例的临床情况探讨Gli2对骨巨细胞瘤预后的影响。结果免疫组织化学结果提示Gli2在骨巨细胞瘤中高表达，其阳性率在恶性病例组[100.0%（6／6）]高于良性病例组[55.0%（22／40）]，差异有统计学意义（P＝0.035）；在复发病例组有增高趋势，但与未复发病例组差异无统计学意义（P＝0.204）。实时PCR结果表明骨巨细胞瘤组织中Gli2 mRNA相对表达量高于正常骨组织（P＜0.0001），其表达水平在恶性病例组、CampanacciⅢ级组及复发病例组有增高趋势，但与其他组相比差异均无统计学意义（均P＞0.05）。 Gli2与甲状旁腺激素相关蛋白（PTHrP）、PTHrP与核因子κB受体活化因子配体（RANKL）、RANKL与骨保护素（OPG）表达具有相关性（均P＜0.05）。结论 Gli2在骨巨细胞瘤中的表达水平增高，可能参与骨巨细胞瘤的复发及恶性转化。其可能通过与PTHrP及RANKL之间的调控关系参与骨巨细胞瘤的溶骨作用。
Objective To investigate the expression of glioma-associated oncogene 2 （Gli2） in giant cell tumor of bone, and analyze the differential expression in the different classification of imaging and pathology and the recurrent diseases, to discuss its role and significance in the pathogenesis and prognosis of giant cell tumor of bone. Methods 46 cases of giant cell tumor of bone who received the primary treatment in Peking University Peopleˊs Hospital during Jan 2009 and Dec 2012 and had the full data of treatment and could be follow-up were collected. Among which there were 40 benign cases and 6 malignant cases. The expression of Gli2 in the different cases of giant cell tumor of bone were detected by immunohistochemistry and real-time PCR, and its effect on the prognosis of giant cell tumor of bone was analyzed. Results Immunohistochemistry revealed positive expression of Gli2 in giant cell tumor of bone. The positive rate of Gli2 in malignant cases was significantly higher than that in benign cases [100.0 % （6/6） vs 55.0 % （22/40）, P=0.035], and only found increasing trend but no significant difference among recurrent cases （P=0.204）. Results of real-time PCR indicated that Gli2 highly expressed in giant cell tumor of bone, and the increasing trend of expression in different pathological types, different image classifications and recurrent cases, but no statistical different were found （all P〈0.05）. The expression correlation analysis showed that the expression of Gli2 and PTHrP, and the expression of RANKL and OPG either had a significant correlation （both P〉 0.05）. Additionally the expression of PTHrP and RANKL had a moderate correlation （P〉0.05）. Conclusions The expression level of Gli2 is high in giant cell tumor of bone, and Gli2 may be involved in recurrence,metastasis and malignant transformation. Gli2 may effect the osteolytic process of giant cell tumor of bone by the＆amp;nbsp;regulation mechanism of Gli2-PTHrP-RANKL pathway.
出处
《肿瘤研究与临床》
CAS
2015年第9期577-581,585,共6页
Cancer Research and Clinic
基金
北京大学人民医院研究与发展基金（RDB2013-08）
