摘要
目的了解宁夏医科大学总医院金黄色葡萄球菌临床耐药及pvl、qac A/B基因携带情况,为MRSA防治提供数据支持。方法用VITEK-2全自动细菌鉴定仪和K-B纸片扩散法对临床分离的1 654株金黄色葡萄球菌进行细菌鉴定及药敏试验,PCR检测MRSA菌株pvl和qac A/B基因,用WHONET 5.6和SPSS 17.0软件进行统计分析。结果本院金黄色葡萄球菌主要来源于脓液(42.5%)和痰液(32.0%);检出率较高的科室为烧伤科(29.8%)、骨科(11.0%)、脑外科(10.0%)和ICU(9.8%);MRSA平均检出率为50.1%,不同年份间MRSA检出率差异有统计学意义;MRSA对多种抗生素耐药性较高,耐药率明显高于MSSA,尚未发现呋喃妥因、利奈唑胺、万古霉素、替考拉宁的MRSA耐药菌株;MRSA基因检测结果显示pvl和qac A/B基因携带率分别为1.23%和0.55%。结论医院应加强对金黄色葡萄球菌感染高发病区的防治工作,同时完善对耐药菌株pvl和qac A/B基因的监测,规范抗生素使用,降低耐药菌株的发生率。
Objective To investigate the clinical drug resistance and the cartier rate of pvl and qacA/B gene of Staphylococcus aureus in Ningxia medical university general hospital, so as to provide scientific basis for the prevention and control of MRSA. Methods 1 654 strains of Staphylococcus aureus were isolated, by VITEK - 2 automatic bacterial identification system and the K- B disk diffusion method, for bacterial identification and susceptibility testing. Polymerase chain reaction（PCR） was used for the detection of pvl and qacA/B genes of MRSA. And the statistical analysis was performed by WHONET 5.6 software and SPSS 17.0 software. Results The Staphylococcus aureus strains isolated from this hospital were mainly the pus（42.5% ） and sputum specimens （ 32.0% ）. The departments with high detection rate were department of bums （ 29.8% ）, orthopedics （ 11.0% ）, neurosurgery （ 10.0% ） and ICU （9.8%）. The average detection rate of MRSA was 50.1%, and the differences of detection rates of MRSA in different years were statistically significant. Drug resistance analysis showed that MRSA had a high resistance to many antibiotics. The resistance rate of MRSA was significantly higher than that of MSSA. No resistance to nitro- furantoin, linezolid, vancomycin and teicoplanin MRSA strain was found. Genetic tests of MRSA strains showed that the carrier rates ofpv/and qacA/B genes were 1.23% and 0.55% respectively. Conclusion We should strengthen the prevention and treatment of Staphylococcus aureus infection in the high incidence areas. Meanwhile it is necessary to consummate the continuous monitoring for pvl and qacA/B genes of resistant strains. Furthmore, we ought to regulate the use of antibiotics to reduce the in- cidence of drug - resistant strains.
出处
《中国卫生检验杂志》
CAS
2015年第18期3183-3186,共4页
Chinese Journal of Health Laboratory Technology
基金
宁夏科技支撑项目(2013)
宁夏回族自治区科技惠民计划(2013)
宁夏临床病原微生物重点实验室开放课题(2015
LCPM201503)
关键词
金黄色葡萄球菌
耐甲氧西林的金黄色葡萄球菌
耐药率
Staphylococcus aureus
Methicillin- resistant Staphylococcus aureus
Drgu resistance rate
