摘要
目的 将微波消融应用于羊胸腺组织，固定消融功率，分析消融灶大小随消融时间变化的趋势，观察消融灶的大体及组织学变化，比较超声下气化范围和实际消融灶大小的关系。方法 新鲜离体羊胸腺50个，羊胸腺最厚处约2 ～ 3 cm。根据消融时间不同分为4组，即30 s组、40 s组、50 s组、60 s组。在超声引导下，利用微波消融治疗仪（含冷循环装置）和发射段长5 mm、直径2 mm的电极，设置消融功率为40 W，对离体羊胸腺组织进行不同时间（30、40、50、60 s）的微波消融，测量气化区长径、消融灶长径及短径，并对消融灶行病理学检查。结果 随着消融时间延长，气化区长径、消融灶长径、短径和消融灶体积均呈现增大趋势；气化区长径与消融灶长径呈线性相关（r = 0.968，P 〈 0.05）；在相同消融时间下，气化区长径小于消融灶长径（P 〈 0.05）；消融灶质地变硬，边界清晰，光学显微镜下消融灶内胸腺组织形态失常，其内淋巴细胞难以辨认。结论 微波消融羊胸腺组织时，固定微波消融功率，消融灶大小随消融时间延长而增大；所得消融灶长径明显大于超声下气化区长径，临床通过气化范围评估消融程度需要慎重。
Objective To analyze the trend of ovine thymus gland ablation lesion size changes with time after microwave ablation at constant power, observe gross and histological changes of foci, and compare the relationship between range of gasification by ultrasound and actual ablation lesions. Methods The 50 isolated fresh ovine thymus glands with thickness about 2- 3 cm were divided into 4 groups accorded to different ablation times： 30-second group, 40-second group, 50-second group and 60-second group. Ablation power was fixed at 40 W, the ovine thymus gland ablation lesions were made by ultrasound-guided microwave ablation with electrode of 2 mm diameter and wave length 5 mm. Microwave ablations were performed for different time lengths（30-second, 40-second, 50-second and 60-second）. The long diameter of gasification, long and short diameters of ablation were measured and the pathology of lesions area was studied. Results The long diameter of gasification, long and short diameter of ablation and lesion area were increased with ablation time. The long diameter of gasification and long diameter of ablation were linear correlated（r = 0.968, P 0.05）. At the same ablation time, the long diameter of gasification area was shorter than that of lesion area（P 0.05）. The thymus gland became harder with well-defined border after ablation, the lymphoid tissue disorder in lesions was observed by light microscopy, while lymphocytes were hard to identify. Conclusion It is demonstrated that at constant power of microwave ablation, the size of gasification and lesions increase with ablation time. The long diameter of lesion area is significantly longer than that of gasification area, so it should be careful to evaluate the area of ablation by gasification region in clinic.
出处
《生物医学工程与临床》
CAS
2015年第5期455-459,共5页
Biomedical Engineering and Clinical Medicine
关键词
羊胸腺
微波消融
气化
消融灶
ovine thymus gland microwave ablation gasification lesion areas
