摘要
目的探讨δ-catenin在肾透明细胞癌(Clear cell renal cell carcinoma,cc RCC)组织中的表达情况,并分析其临床意义。方法采用免疫组化SP法和Western blot检测98例cc RCC组织及40例癌旁组织中δ-catenin蛋白的表达;应用Real-time PCR检测20例cc RCC及癌旁新鲜组织中δ-catenin m RNA的表达;分析δ-catenin蛋白表达与临床病理参数及预后的关系。结果δ-catenin m RNA和蛋白在cc RCC中表达水平显著高于癌旁正常组织(<0.05)。δ-catenin蛋白的阳性表达与cc RCC患者性别、淋巴结转移及血行转移相关(<0.05)。δ-catenin蛋白高表达的患者预后明显差于低表达的患者(<0.05)。COX多因素回归分析显示,δ-catenin蛋白表达水平是影响cc RCC患者预后的独立危险因素。结论δ-catenin在cc RCC发生发展中发挥重要作用,可作为cc RCC预后判断的生物标志物。
Objective To investigate the expression and significance of δ-catenin in human clear renal cell carcinoma（ccRCC）. Methods The expression of δ-catenin protein（in 98 cases of ccRCC tissues and 40 cases of adjacent normal tissues） and mRNA（in 20 cases of ccRCC and adjacent normal tissues） was detected by Western blot and immunohistochemistry and Real-time PCR respectively, the relationship of δ-catenin protein expression with clinicopathological parameters and prognosis was analyzed. Results The expression levels of δ-catenin protein and mRNA was significantly higher in ccRCC tissue than in adjacent normal tissues（P〈0.05）. The expression of δ-catenin protein was closely related to gender, lymph node metastasis and vascular invasion（P〈0.05）. The prognosis of patients with high expression of δ-catenin protein was significantly worse than the patients with low expression（P〈0.05）. Multivariate COX regression analysis showed that δ-catenin protein was an independent prognostic factor for patients with ccRCC. Conclusion δ-catenin plays an important role in the development of ccRCC, could be a biomarker for judge of the prognosis of ccRCC.
出处
《解剖科学进展》
CAS
2015年第5期463-467,共5页
Progress of Anatomical Sciences
基金
辽宁省自然科学基金(No.20092028)
