摘要
目的研究mi R-421在胃癌BGC-823细胞凋亡过程中的分子机制。方法通过si RNA转染技术将mi R-421 mimics与mi R-421 inhibitors转染BGC-823胃癌细胞;Western blotting方法分析细胞凋亡相关蛋白caspase-3、Bax、Bcl-2以及细胞凋亡受体蛋白TNFR-Ⅰ、TNFR-Ⅱ的表达水平。结果 mi R-421 mimics与mi R-421 inhibitors成功转染BGC-823胃癌细胞;caspase-3、Bax蛋白在mi R-421 inhibitors转染细胞中表达增强,而Bcl-2、TNFR-Ⅰ与TNFR-Ⅱ则表达降低。结论 mi R-421在BGC-823胃癌细胞中可能通过调控肿瘤细胞凋亡相关蛋白caspase-3、Bax、Bcl-2的表达及肿瘤细胞表面凋亡受体蛋白TNFR的表达从而影响BGC-823的凋亡。
Objective To study the molecular mechanism of miR-421 in the apoptosis of BGC-823 gas-tric cancer cells. Methods miR-421 mimics and miR-421 inhibitors were transfected into BGC-823 cells by siRNA transfection technology. The expression of proteins related to apoptosis or receptors such as caspase-3, Bax, Bcl-2, TNFR-Ⅰand TNFR-Ⅱwere detected by Western blotting. Results The miR-421 mimics and miR-421 inhibitors were transfected into BGC-823 cells successfully. The expression of caspase-3 and Bax proteins were en-hanced in BGC-823 cells transfected with miR-421 inhibitors, while the expression of Bcl-2, TNFR-Ⅰand TNFR-Ⅱwere decreased. Conclusion The expression of apoptosis proteins caspase-3, Bax, Bcl-2 and cell surface recep-tor proteins TNFR are regulated by miRNA-421 to influence the apoptosis of BGCe-823 gastric cancer cells.
出处
《海南医学》
CAS
2015年第17期2500-2502,共3页
Hainan Medical Journal
基金
昆山市科计划项目(编号:KS1347)
