摘要
目的探讨抑制骨髓间充质干细胞(BMSCs)成脂分化能力对骨质疏松(OP)的早期疗效。方法选取雌性SD大鼠40只,采用背侧入路摘除卵巢法建立大鼠OP模型。随机分为4组:A组(假手术组)、B组(模型组)、C组(BMSCs治疗组)、D组(RNAi转染BMSCs治疗组),每组10只。造模后7 d经尾静脉分别注射生理盐水、10%胎牛血清的LG-DMEM培养液、BMSCs、RNAi转染BMSCs,BMSCs来自课题组从SD大鼠骨髓中提取并经过流式细胞仪和诱导分化染色鉴定的BMSCs,RNAi转染BMSCs来自课题组前一部分体外实验中使用腺病毒转染后的BMSCs。干预后,依照分组行骨密度(BMD)、骨转换标志物检测。比较4组大鼠的骨转换标志物在OP早期骨形成与骨吸收过程中的变化。结果 D组的BMD显著高于B、C组(P<0.05),而与A组差异无统计学意义(P>0.05);D组的PⅠNP高于B、C组(P<0.05),而与A组差异无统计学意义(P>0.05);D组的β-CTX低于B、C组(P<0.05),而与A组差异无统计学意义(P>0.05)。结论 RNAi转染BMSCs可提高去势大鼠的BMD,促进骨形成。
Objective To explore the early curative effect of restraining fat differentiation ability of bone marrow mesenehymal stem cells （BMSCs） in osteoporosis （OP）. Methods 40 female SD rats were selected.Osteoporotic of rats model was established by dorsal approach ovary removal method.Rats were randomly divided into four groups：group A （shamoperated group）,group B （model group）,group C （BMSCs treatment group） and group D （RNAi transfection BMSCs treat- ment group）.Each group has 10 rats.After 7 days moldeling,rats in four groups were injected saline,LG-DMEM culture contained 10% fetal bovine serum,BMSCs and RNAi transfection BMSCs.BMSCs were extracted from SD rat bone marrow of and identified by flow cytometer and induced differentiation dyeing.RNAi transfection BMSCs were from the transfeeted BMSCs of adenovirus used for the former parts of in vitro experiment in research group.After intervention, bone mineral density （BMD） and markers of bone turnover were tested according to grouping.The change of bone turnover markers of rats in bone formation and bone resorption of the early osteoporosis was compared among four groups. Results BMD in group D was higher than that of group B and C （P〈0.05）,but had no significant difference compared with group A （P〉0.05）.PINP in group D was higher than that of group B and C （P〈0.05）,but had no significant difference compared with group A （P〉0.15）.I3-CTX in D group was lower than that of group B and C （P〈0.05）,but had no significant difference compared with group A （P〉0.05）. Conclusion RNAi transfection BMSCs can improve BMD of castration rats and promote bone formation.
出处
《中国当代医药》
2015年第27期14-16,共3页
China Modern Medicine
关键词
RNA干预
骨髓间充质干细胞
骨质疏松
骨密度
Ⅰ型前胶原氨基端前肽
β-Ⅰ型胶原羧基端肽
RNA interference
Bone marrow mesenehymal stem eells
Osteoporosis
Bone mineral density
Procollagen type I N-terminal propeptide
β-C-terminal cross-linking telopeptide of type I collagen
