摘要
目的:研究京万红组方成分中盐酸小檗碱、黄芩苷及冰片的体外抗白色念珠菌作用。方法:以白色念珠菌为研究对象,K-B纸片扩散法分别测定50,100,150 g·L-1的盐酸小檗碱、黄芩苷、冰片及制霉菌素药液抑菌圈直径;采用试管双倍稀释法和棋盘法,测定盐酸小檗碱、黄芩苷、冰片、制霉菌素分别最低抑菌浓度(MIC)和最低杀菌浓度(MBC),测定盐酸小檗碱、黄芩苷、冰片两两联用及3种组分联用的MIC,计算出联合抑菌分数(FIC)。结果:盐酸小檗碱、黄芩苷、冰片MIC分别为160,1 280,320 mg·L-1;MBC分别为640,20 480,640 mg·L-1。盐酸小檗碱与黄芩苷联用FIC指数0.5,为协同作用;黄芩苷与冰片联用FIC指数为0.625,为相加作用;盐酸小檗碱与冰片联用FIC指数为0.5,为协同作用。3种组分药物联合使用FIC指数为0.375,有一定的协同作用。结论:盐酸小檗碱、黄芩苷、冰片对白色念珠菌均有抑制作用,且3种组分药物联合使用具有一定的协同作用。
Objective： To study the effect of anti-Candida albicans of Jingwanhong constituents of berberine hydrochloride, baicalin and borneol in vitro. Method： The minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of berberine hydrochloride, baicalin, borneol （50, 100, 150 g .L-1 ） were determined by two-fold dilution method and checkboard method. The anti-bacterial effects of the combined three drugs were measured and the combined inhibitory fraction （FIC） was calculated. Result： The MIC of berberine hydrochloride, baicalin, borneol was 160, 1 280, 320 mg .L-1, and the MBC was 640, 20 480, 640 mg -L-1, respectively. Baicalin and berberine hydrochloride combined showed the synergistic effect with FIC index of 0. 5. The FIC of baicalin and borneol combined was 0. 625, which showed the additive effect. The berberine hydrochloride and borneol combined showed the synergistic effect with FIC index was 0.5. Three-drug combination had a certain synergy with the FIC index of 0. 375. Conclusion： Three constituents of berberine hydrochloride, baicalin, borneol could inhibit C. albicans, and three drugs combined showed a certain synergy.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第19期111-115,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
科技型中小企业发展专项(xqk2010-008)
关键词
白色念珠菌
盐酸小檗碱
黄芩苷
冰片
Candida albicans
berberine hydrochloride
baicalin
borneol
