摘要
建立快速鉴别不同产地珠子参的方法,为其质量评价提供依据。采用UV指纹图谱结合PLSDA法鉴别不同产地的珠子参样品,分析珠子参的UV特征光谱存在的差异,利用PLS-DA方法分析不同珠子参样品UV特征光谱的吸光度值。结果表明,不同产地珠子参样品的UV指纹图谱的特征吸收峰的吸收波长相似,峰强度存在差异,能有效区别不同产地的珠子参样品;PLS-DA方法能把怒江、玉溪、大理和文山的珠子参样品分为4类,除了大理和文山样品相近,其余产地样品相距较远。
To establish a rapid method for the identification of Panax japonicus var.major（ Araliaceae） from different geographical origins and provide the basis for quality evaluation,UV spectra combined with partial least squares discriminant analysis（ PLS-DA） method was used to distinguish P.japonicus var. major samples from different cultivation regions.Then the difference of P.japonicus var.major in UV spectra was analyzed, and the absorbance was investigated by PLS-DA.The results indicated that the ab-sorption wavelength of the UV fingerprint of P.japonicus var.major samples from different regions was similar, and the peak intensity was different, which could effectively distinguish the P.japonicus var. major samples from different geographical origins.The PLS-DA method successfully separated the samples from Nujiang,Yuxi,Dali and Wenshan.Moreover,samples collected from Dali and Wenshan were similar, while the remaining samples were far from each other.
出处
《河南农业科学》
CSCD
北大核心
2015年第9期91-94,共4页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金(81260610
81260608)
科技部"十二五"国家科技支撑计划项目(2011BAI13B01-02
2011BAI13B02-04)
农业部公益性行业科研专项(201303117)
云南省科技计划项目(2012CG024)
关键词
珠子参
紫外指纹图谱
PLS-DA
产地鉴别
Panax japonicus var. major
UV spectra fingerprint
PLS-DA
identification of origin
