摘要
目的初步研究IL-25通过IL-4和IL-13促进哮喘小鼠早期气道重塑,探讨IL-25在早期哮喘气道重塑中的作用。方法将40只BALB/C雌性小鼠随机均分为哮喘组、对照组、IL-25组和抗IL-25组,哮喘组、IL-25组和抗IL-25组是用鸡卵白蛋白(OVA)激发和致敏建立哮喘模型,其中IL-25组、抗IL-25组分别于每次致敏前1 h鼻腔内滴入重组小鼠IL-25、抗重组小鼠IL-25,对照组是用0.9%生理盐水替代。用酶联免疫吸附试验(ELISA)测定血清和肺泡灌洗液中IL-4和IL-13的表达,用HE染色观察各组气道基底膜厚度,用免疫组织化学法测定气道中α-平滑肌肌动蛋白(α-SMA)的表达量(气道基底膜厚度和α-SMA均为气道重塑的重要指标)。结果 ELISA显示:与哮喘组相比,在血清和肺泡灌洗液中IL-25组的IL-4和IL-13表达量明显增加(P<0.05),抗IL-25组中IL-4和IL-13表达量明显降低(P<0.05);HE染色显示:与哮喘组相比,IL-25组支气管管壁增厚明显、管腔狭窄严重,气道炎性细胞浸润明显增加,黏膜上皮破损严重,抗IL-25组管壁厚度、管腔狭窄、炎症细胞浸润及黏膜上皮破损较哮喘组明显减轻;免疫组织化学的结果显示:与哮喘组相比,IL-25组支气管壁α-SMA蛋白表达明显增加(P<0.05),而抗IL-25组α-SMA蛋白表达则明显减少(P<0.05)。结论 IL-25通过IL-4和IL-13促进气道上皮细胞的增生,炎性细胞的聚集、浸润,黏液分泌物的增加,可能对哮喘小鼠早期气道重塑起到一定的促进作用。
ObjectiveTo explore the role of IL-25 in early airway remodeling of asthma on the regulation of IL-4 and IL-13.MethodsSerum and BALF were colected from forty BALB/C female mice which were randomly divided into asthma group, control group, IL-25 group and anti IL-25 group. Asthma model was established by inhaling chicken ovalbumin （OVA） in asthma group, IL-25 group and anti IL-25 group. One hour before every sensitization, IL-25 restructuring mice and anti IL-25 restructuring mice were respectively dripped into the noses of the IL-25 group and anti IL-25 group, while the control group with 0.9% saline instead. The level of IL-4 and IL-13 in serum and BALF were determined by ELISA. Basement membrane thickness as an important index of airway remolding was detected by HE staining. The expressing ofα-SMA was determined by immnohistochemistry.ResultsELISA showed that compared with the asthma group, the expression of IL-4 and IL-13 of the serum and BALF of IL-25 group increased significantly （P〈0.05）, while the expression in anti IL-25 groups decreased obviously （P〈0.05）.The HE staining showed that compared with the asthma group, the thickness of bronchial waland the stenosis of bronchial lumen and the airway inflammatory cels infiltration and the damage of mucosal epithelium of IL-25 group increased obviously. While the thickness of bronchial wal and the stenosis of bronchial lumen and the airway inflammatory cels infiltration and the damage of mucosal epithelium of anti IL-25 group decreased significantly. The immunohistochemical showed that compared with the asthma group, the expression of α-SMA of bronchial wal of IL-25 group increased significantly （P〈0.05）, while the expression in anti IL-25 groups decreased obviously （P〈0.05）.ConclusionIL-25 has the potential to promote airway remoding through IL-4 and IL-13, which can promote the hyperplasia of the airway epithelial cels, the accumulation of inflammatory cels, infiltration, mucus secretion increased.
出处
《中华临床医师杂志（电子版）》
CAS
2015年第15期58-61,共4页
Chinese Journal of Clinicians(Electronic Edition)
基金
山东省科技攻关课题(2012GSF11830)
山东省自然科学基金(ZR2013HM046)
