摘要
含硫氨基酸具有动物营养与免疫相关的重要生理功能,为提高菊苣中含硫氨基酸含量,采用根癌农杆菌介导法将玉米种子贮藏蛋白含硫氨基酸基因γ-zein和绿色荧光蛋白GFP融合基因转入到菊苣无菌苗叶片中,经过共培养、潮霉素抗性筛选、分化、再生和炼苗,得到抗性植株。对抗性植株进行PCR、PCR-Southern、斑点杂交和RTPCR分析,结果表明,外源目的基因已经整合到菊苣基因组中并且得到了表达,为提高菊苣含硫氨基酸含量,改善其品质奠定了基础。
Sulfur-containing amino acids have important physiological functions related to animal nutrition and immunity.To improve the sulfur-amino acid content of chicory,leaves of chicory were transformed with the Sulphur-amino acid gene γ-zein,an important prolamin storage protein from Zea mays and a green fluorescent protein (GFP)gene using Agrobacterium mediated transfusion.After co-culture,selective differentiation and regeneration,hygromycin resistant plants were obtained.Resistant plants were detected using PCR,PCR-southern,dot blot hybridization and RT-PCR.The results demonstrated that the γ-zein genes had been inte-grated into the genome of chicory and expressed on a nucleic acid level in the transgenic plants.
出处
《草业学报》
CSCD
北大核心
2015年第9期73-88,共16页
Acta Prataculturae Sinica
基金
四川省十二五牧草育种攻关(2011NZ0098-11)
四川省应用基础项目(2013JY0111)
国家牧草产业技术体系阿坝综合试验站资助
关键词
γ-zein基因
菊苣
农杆菌转化
含硫氨基酸
γ-zein genes
chicory(Cichorium intybus)
agrobacterium-mediated transformation
sulphur-amino acid
