摘要
为比较烤烟组培苗与常规苗烟苗素质及大田期的抗性差异,以烤烟品种(系)K326、粤烟97、华烟06的组培苗与常规苗为试验材料,测定了苗期农艺性状及根系活力和大田期多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、抗坏血酸过氧化物酶(APX)、β-1,3-葡聚糖酶等防御性酶活性,并调查了田间发病率。结果表明:组培苗在株高、叶数、茎围、根系体积、根系活力、干鲜重等方面均优于常规苗。在整个大田生育期内,不同处理烤烟的防御酶活性基本呈现出上升—下降—上升的变化趋势。旺长期前,组培苗与常规苗之间防御酶活性差异不大,旺长期受到病虫害侵染后,各处理的防御酶活性均先下降然后上升,并在较长一段时间内处于较高水平,且组培苗防御酶活性增加的幅度高于常规苗,其中K326组培苗的PPO、PAL、β-1,3-葡聚糖酶活性分别比常规苗高出29.58%、15.30%、73.03%,华烟06组培苗APX活性高于常规苗58.43%,差异显著。组培苗的花叶病、气候斑病及虫害发生率等均低于常规苗,表现出较强的抗性。
Resistance capability difference between tissue culture seedlings and conventional seedlings was studied by using varieties（strains） K326,Yueyan97,and Huayan06,whose root activity and agronomic characters in seedling period,incidence rate and activities of PPO,PAL,APX,β-1,3-Glucanase during field period were investigated.Results indicated that tissue culture seedlings outperformed conventional seedlings in terms of plant height,leaf number,stem girth,root volume,root activity and dry weight.Defensive enzyme activity was in the pattern of up-down-up,which meant that it increased at the start,then decreased and rose again in the final period.Tissue culture seedlings showed no significant difference with conventional seedlings before vigorous growth stage.The enzyme activities decreased before increasing when infected by diseases and maintained at a high level for a long period.The increase of enzyme activities of tissue culture seedlings was higher than that of conventional seedlings.In particular,PPO,PAL and β-1,3-Glucanase of tissue culture seedling K326 was 29.58%,15.30%,73.03% higher than that of conventional seedlings respectively and Huayan06 tissue culture seedling＇s APXA was 58.43% higher than that of conventional seedlings.The tissue culture seedling proved to have advantage in disease-resistance and insect-resistance.
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2015年第4期70-77,共8页
Acta Tabacaria Sinica
基金
中国烟草总公司广东省公司科技项目(201104
粤烟科【2011】28号)
关键词
烤烟
防御酶
组培苗
常规苗
flue-cured tobacco
defensive enzymes
tissue culture seedling
conventional seedling
