摘要
为了发展一种将150 kb及以上的外源DNA片段引入变铅青链霉菌（Streptomyces lividans）中的有效方法,以大肠杆菌-变铅青链霉菌穿梭细菌人工染色体为载体,将携带完整格尔德霉素生物合成基因簇的3个150-180 kb的外源DNA片段期望以接合转移的方式从大肠杆菌宿主菌（Escherichia coli ET12567/p UZ8002）中横向转移入变铅青链霉菌（Streptomyces lividans）TK23菌株中。结果表明,接合转移技术能够有效地将携带完整格尔德霉素生物合成基因簇的3个外源DNA大片段引入到变铅青链霉菌基因组中并稳定传代。
In order to develop an efficient method of transferring 150 kb and above foreign DNA fragments into Streptomyces lividans, intergenerie conjugation technology was utilized to transfer three 150-180kb foreign DNA fragments, carrying whole geldanamyein biosynthesis gene cluster, into S. lividans TK23 genome, with bacterial artificial chromosome （BAC） of Es- cherichia coli ET12567/pUZ8002-S. lividans TK23 as vectors. The result showed that all the three foreign DNA fragments were efficiently transferred into S. lividarts TK23, and were steadily inherited.
出处
《湖北农业科学》
2015年第15期3776-3778,3783,共4页
Hubei Agricultural Sciences
基金
广东省自然科学基金博士启动项目(S2012040006215)
广东省中国科学院全面战略合作项目(2012B091100276)
