摘要
建立了同时测定祛痘类化妆品中3种禁用除螨剂（克霉丹、灭螨猛和克螨特）的高效液相色谱分析方法及质谱确证方法。化妆品样品以甲醇为提取溶剂进行超声提取,提取液离心处理,取上清液经微孔滤膜过滤后测定。采用Kromasil C18（4.6 mm×250 mm,5μm）色谱柱进行分离,外标法定量。祛痘类化妆品中克霉丹、灭螨猛和克螨特的方法定量限分别为0.05,0.005和0.02 mg/kg,在低、中、高3个添加水平范围内平均回收率为80.7%～110.4%,相对标准偏差为2.3%～10%。采用高效液相色谱-串联质谱法进一步确证分析,电喷雾正离子模式电离,多反应监测模式检测。该方法适用于祛痘类化妆品的实际检验工作。
A comprehensive analytical method based on high-performance liquid chromatography （HPLC） has been developed for the simultaneous determination of three anti-acarid agents （captan, chinomethionate and propargite） in anti-ache cosmetics. Cosmetic samples were ultrasonically extracted with methanol, centrifuged, and filtered by microporous membrane filter. The chromatographic separation of the analytes was performed using a Kromasil C18 （4. 6 mm× 250 ram, 5 μm）column. The quantitation was carried out by external standard method. The experimental results indicated that the limits of quantitation （LOQs） for captan, chinomethionate and propargite were 0.05, 0. 005 and 0.02 mg/kg, respectively. The suspected positive samples were subjected to further verification by HPLC-MS/MS. Positive electrospray （ESI） and multiple reaction monitoring （MRM） were used for the analysis. The method is accurate, rapid, sensitive, and can be used for the inspection of anti- acne cosmetics.
出处
《分析试验室》
CAS
CSCD
北大核心
2015年第9期1038-1041,共4页
Chinese Journal of Analysis Laboratory
基金
质检公益性行业科研专项项目(2012104013-4)
国家科技支撑计划项目(2013BAK04B03)资助
关键词
祛痘类化妆品
除螨剂
高效液相色谱-串联质谱
Anti-aene cosmetics
Anti-acarid agents
High-performance liquid chromatography tandemmass spectrometry
