摘要
目的：研究LDYS-14007对JAK1-STAT3信号通路的影响。方法分别用10μmol 、1 nmol 的LDYS-14007和10μmol的Tofacitinib处理MDA-MB-231细胞。用Western blot法检测JAK1和STAT3的蛋白表达以及磷酸化状况。结果吸光度A与蛋白质浓度C呈线性相关，线性方程为A＝0.0075C＋0.0029，r＝0.9976，线性范围为1.08～5.08 mg／mL；随着LDYS-14007浓度的增加， Phospho-JAK1，Phospho-STAT3的量逐渐减少。结论 LDYS-14007可通过降低JAK1和STAT3的磷酸化水平抑制JAK1-STAT3信号通路。 LDYS-14007可能在类风湿性关节炎（ rheumatoid arthritis， RA）的治疗中起重要作用。
Objective To study the effect of LDYS-14007 on JAK1-STAT3 signaling pathways.Methods MDA-MB-231 cells were treated with 10μmol,1 nmol LDYS-14007, and 10 μmol Tofacitinib,respectively.Western blot assay was used to determine the expression of JAK1,Phospho-JAK1, STAT3 and Phospho-STAT3.Results The absorbance value was linearly related to the concentration of protein C, The linear equation is A=0.0075C＋0.0029, r=0.9976, The linear range of 1.08-5.08 mg/mL, With the increased concentration of LDYS-14007, the amount of Phospho-JAK1, Phospho-STAT3 were all gradually decreased.Conclusion LDYS-14007 leads to the levels of Phospho-JAK1 and Phospho-STAT3 decrease, which inhibits JAK1-STAT3 signaling pathway.LDYS-14007 may play an important role in the treatment of rheumatoid arthritis.
出处
《中国生化药物杂志》
CAS
2015年第6期10-12,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
国家高新技术研究发展计划(863计划
2012AA02A306)
