摘要
目的探讨甘草酸（glycyrrhizin，Gly）对1型糖尿病（T1DM）脑缺血／再灌注损伤大鼠神经功能恢复的影响。方法应用链脲佐菌素（streptozotocin，STZ）诱导建立雄性SD大鼠1型糖尿病模型，诱导14天后进行大脑中动脉缺血／再灌注损伤（middlecerebralartery occlusion，MCAo）模型制备。脑缺血／再灌注后24h，分别经尾静脉给予PBS、Gly1ml（4mg／kg）。实验大鼠随机分为3组（每组n=6）：正常大鼠脑缺血／再灌注对照组（N—MCAo组）、T1DM大鼠脑缺血／再灌注损伤组（T1DM—MCAo组）、T1DM大鼠脑缺血／再灌注损伤Gly治疗组（T1DM—MCAo＋Gly组）。术后1、7、14天分别对各组大鼠进行神经功能损伤评估。结果术后第1天，各组改良神经功能缺损评分（mNSS）、足错步率比较差异无统计学意义（P〉0．05）；术后第7、14天，与N—MCAo组相比，TIDM—MCAo组mNSS明显升高（P〈0．05）；与T1DM—MCAo组相比，T1DM—MCAo＋Gly组大鼠mNSS明显降低（P〈0．05）。结论Gly促进T1DM脑缺血／再灌注损伤大鼠神经功能恢复。
Objective To investigate the effects of glycyrrhizin （Gly） on the restoration of nervous functions in type 1 diabetic rats with cerebral ischemia/reperfusion injury. Methods A model of type 1 diabetic rats was established through injection of streptozotocin. Two weeks later, all rats were subjected to middle cerebral artery occlusion （MCAo） for 2 h. One day later, the rats were injected with 1 ml of PBS or Gly （4 mg/kg） via the tail vein. The rats were divided into three groups （n =6） ： a normal control group （Group N -MCAo）, a MCAo group （Group T1Dm -MCAo） and a Gly treatment group （Group T1DM -MCAo ＋ Gly）. Then, the nervous functional injuries in rats were assessed on Days 1, 7 and 14 after operation. Results No statistical difference was measured as to the modified Neurological Severity Score （mNSS） and wrong step rate among the groups on Day 1 （P 〉 0.05）. On Days 7 and 14, Group T1DM - MCAo produced substantial decreases in mNSS than Group N - MCAo （P 〈 0.05 ）, which were reduced after Gly treatment （P 〈 0. 05）. Conclusion Gly treatment can stimulate the recovery of functional outcomes in type 1 diabetic rats with ischemia/reperfusion injury in brain.
出处
《徐州医学院学报》
CAS
2015年第7期437-440,共4页
Acta Academiae Medicinae Xuzhou
基金
国家自然科学青年基金（16611214）
关键词
1型糖尿病
脑缺血/再灌注
神经保护
甘草酸
type 1 diabetes
cerebral ischemia/reperfusion
neuroprotection
glycyrrhizin
