摘要
目的：考察替米沙坦对体外培养的人脐带动脉平滑肌细胞（HUASMCs）增殖的抑制作用及对白细胞介素-6（IL-6）表达的影响。方法：组织块贴壁法,HUASMCs原代培养,传3代细胞实验。MTT比色法检测同一浓度替米沙坦（50μg·ml-1）在不同作用时间（3～96 h）和不同浓度替米沙坦（0.5～500μg·ml-1）在同一作用时间（48 h）,对HUASMCs增殖的抑制作用。RT-PCR和ELISA法检测低、中、高3种浓度的替米沙坦（1,10,100μg·ml-1）对HUASMCs表达IL-6 mRNA和因子的影响。结果：不同作用时间的替米沙坦（50μg·ml-1）对HUASMCs的增殖有抑制作用（P〈0.05）,作用时间为48 h时,抑制作用最强,抑制率为（29.12±2.89）%。其后的作用时间72 h和96 h的抑制作用与48 h比无显著变化（P〉0.05）。不同浓度的替米沙坦对HUASMCs增殖有抑制作用（P〈0.05）,当浓度为100μg·ml-1时,抑制作用最强,抑制率为（41.46±3.36）%。其后各浓度的抑制作用与100μg·ml-1比无显著变化（P〉0.05）。低、中、高3种浓度的替米沙坦对HUASMCs表达的IL-6mRNA和IL-6含量均有抑制作用（P〈0.05）,并呈浓度依赖性（P〈0.05）。结论：替米沙坦抑制体外培养的HUASMCs增殖,对HUASMCs表达的IL-6 mRNA和因子分泌均有抑制作用。
Objective： To investigate the effects of telmisartan on the proliferation of human umbilical arterial smooth muscle cells （HUASMCs） and the expression of interleukin-6 （IL-6）. Methods： HUASMCs were primarily cultured with a tissue adherent method and the third generation cells were used for the following experiments. MTr assay was employed to detect the inhibitory role of telmisar- tan in the proliferation of HUASMCs with the same concentration （50 μg · ml-1 ） in different duration （ 3 h-96 h） and the different concentrations of telmisartan （0.5-500 μg · ml-1 ） in the same duration （48 h）. RT-PCR and ELISA assay were used to determine the influence of low, medium and high concentration of telmisartan （ t, 10 and 100 μg · ml- 1 ） on the mRNA and protein expression of IL- 6 in HUASMCs. Results： Telmisartan （50 μg · ml - 1 ） inhibited the proliferation of HUASMCs （ P 〈 0.05 ） in a time-dependent man- ner, and the inhibition rate was （29.12 ± 2.89） %, which the strongest inhibition was shown in 48 h. With the subsequent extension of time （72-96 h）, no significant change was found（P 〉0.05）. Different concentrations of telmisartan （0.5-500 μg · ml-1 ） also in- hibited the proliferation of HUASMCs （P 〈 0.05 ）, and the strongest inhibition was shown when the concentration was 100 μg · ml- with the inhibition rate of （41.46 ± 3.36） %. With the increase of the concentration （200-500 μg · ml -1 ）, no significant change of the inhibition rate was shown （P 〉0.05）. Telmisartan （ 1, 10 and 100 μg · ml-2 ） inhibited the expression of IL-6 in HUASMCs in a dose-dependent manner （P 〈 0.05 ）. Conclusion： Telmisartan can inhibit the proliferation of HUASMCs and downregulate the expres- sion of IL-6.
出处
《中国药师》
CAS
2015年第9期1465-1468,共4页
China Pharmacist
基金
黑龙江省政府博士后启动基金项目(编号:LRB05-230)
黑龙江省卫生计生委科研课题(编号:2014-324)
