摘要
目的:研究慢性乙型肝炎患者乙型肝炎病毒(hepatitis B virus,HBV)DNA载量与血清e抗原(HBeAg)的相关性。方法:收集慢性初治乙肝患者330例及无症状HBsAg阳性HBV携带者98例,采用ELISA(enzyme-linked immunosorbent assay,ELISA)法检测HBeAg的含量,实时荧光定量PCR法检测HBV-DNA含量。结果:330例慢性初治乙肝患者HBeAg阳性214例(64.85%),HBV DNA载量的对数值为(6.542±1.128);阴性116例(35.15%),HBV-DNA载量的对数值为(5.611±1.037),HBeAg阳性和阴性患者HBV DNA载量比较差异具有统计学意义(P<0.0001);在HBeAg阳性的患者中,血清HBeAg含量越高,其血清HBV-DNA载量的对数值也越高(P<0.05);98例HBV携带者中,63人HBeAg阳性,血清HBV DNA载量的对数值为(7.021±1.643);35人HBeAg阴性,血清HBV-DNA载量的对数值为(4.272±0.608),两者相比差异有统计学意义(P<0.0001);在HBeAg阳性的HBV携带者中,血清HBeAg含量越高,HBV DNA载量越高(P<0.05)。结论:HBeAg水平能反映慢性初治患者及HBV携带者的血清HBV DNA载量。
Objective： To study the correlation between serum HBV DNA and HBeAg in chronic hepatitis B patients. Methods：A total of 330 treatment-na ve patients with chronic hepatitis B （CHB） and 98 asymptomatie HBV carriers were enrolled in this study. Enzyme-linked immunosorbent assay was used to quantify HBeAg and realtime fluorescence quantitative PCR （RTFQ-PCR） were used to detect serum HBV DNA loads. Results： Among 330 treatment-na ve CHB patients, 214 （64. 850/oo） were HBeAg positive, with a HBV DNA log value of 6. 542±1. 128% 116 （64.85%） were HBeAg negative, with a HBV DNA log value of 5.611±1. 037; the difference of HBV DNA between HBeAg positive and negative patients were stati cally significant （t= 7. 361, P〈0. 0001）. Among those HBeAg positive patients, the quantity of HBeAg was correlated to serum HBV DNA loads. Among the 98 asymptomatic HBV carriers, 65 HBeAg positive carriers with a HBV DNA log value of 7. 021± 1. 643, significantly higher than HBeAg negative carriers （t=9. 525, P〈0. 0001）. Moreover, the quantity of HBeAg was correlated to serum HBV DNA loads in HBeAg positive HBV carriers. Conclusions： The quantity of HBeAg in treatmentna ve CHB patients and asymptomatie HBV carriers can reflect HBV DNA loads.
出处
《海南医学院学报》
CAS
2015年第10期1342-1344,共3页
Journal of Hainan Medical University
基金
广州市医药卫生科技项目(201234124541)~~
