摘要
目的探讨高速离心法、稀释法、乙醚萃取法消除血脂干扰的效果,为消除血脂对生化检测的干扰提供参考。方法收集100例血脂标本,将标本分为4份,每组25份,其中3组分别给予高速离心法、稀释法、乙醚萃取法处理,另1组原血浆检测。比较处理前后各组间的差异,并对高速离心法、稀释法、乙醚萃取法组采用方差分析。结果 3种方法对Ca2+、尿酸(UA)、肌酐(Cr)、血糖(GLU)、清蛋白(ALB)、总蛋白(TP)、直接胆红素(DBIL)、总胆红素(TBIL)、淀粉酶(AMY)、肌酸激酶同工酶(CK-MB)、肌酸激酶(CK)、羟丁酸脱氢酶(HBDH)、乳酸脱氢酶(LDH)、谷氨酰基转移酶(γGT)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)检测值处理前后,差异有统计学意义(P<0.05),检测值由高到低依次为血清原液检测、离子水稀释法、低温高速离心法、乙醚萃取法。结论乙醚萃取法对蛋白质类检测项目影响明显,离子水稀释法受其影响最低,在临床工作中3种方法可联合使用。
Objective To investigate the effects of high-speed centrifugation, dilution and ether extraction methods in eliminating the blood lipid interference to provide reference for eliminating this interference. Methods 100 cases of lipid samples, the specimens were divided into 4 parts, 1 part of each group, three groups were given high-speed centrifugation, dilution and ether extraction methods, and another group was detected as the original plas- ma. The differences before and after processing were compared among groups. The high-speed centrifugation,dilution and ether extraction methods adopted the variance analysis. Results Ca^2＋ , UA, Cr, GLU, ALB, TP, DBIL, TBIL, AMY, CK-MB,CK, HBDH, LDH, 7GT, AST and ALT values detected by 3 kinds of method had statistically signifi- cant differences between before and after processing （P〈0.05）. The detection value in descending order of serum dope testing, deionized water dilution, low-speed centrifugation, ether extraction. Conclusion The ether extraction method significantly affects the protein detection items, the influence of ionized water dilution is minimum, three kinds of method can be combined for use in clinical work.
出处
《检验医学与临床》
CAS
2015年第16期2406-2407,共2页
Laboratory Medicine and Clinic
关键词
高脂血症
生化测定
干扰
hyperlipidemia
biochemical measurement
interference
