摘要
目的探讨miRNA-7及黏着斑激酶（FAK）在结直肠癌中的表达及其临床意义。方法收集结直肠癌及其癌旁结直肠组织各60份，采用免疫组织化学法检测FAK表达，实时荧光定量PCR检测miRNA-7表达。统计学处理采用卡方检验，相关性采用Spearman相关性分析。结果FAK蛋白在结直肠癌中的表达阳性率为75．0％（45／60），在癌旁组织中的表达阳性率为26．7％（16／60），差异有统计学意义（χ^2=28．04，P〈0．01）。磷酸化FAK（p-FAK）蛋白在结直肠癌中的表达阳性率为65．0％（39／60），在癌旁组织中的表达阳性率为21．7％（13／60），差异有统计学意义（χ^2=22．94，P〈0．01）。miRNA-7在结直肠癌组织的表达较癌旁组织下调（P=0．044）。miRNA-7的表达与结直肠癌患者的淋巴结转移呈负相关（r=-2．290，P=一0．022），与结直肠癌患者的TNM分期呈显著负相关（r=-2．698，P=0．007）；但与患者的年龄、性别、肿瘤位置和肿瘤分化程度均无关；miRNA-7表达与FAK、p-FAK表达呈负相关（r=-0．303，P=0．019；r=-0．267，P=0．038）。结论miRNA-7可能通过调控FAK的表达参与结直肠癌的发生与发展，为结直肠癌的诊疗提供新的靶点。
Objective To investigate the expression and clinical significance of microRNA-7 （miRNA-7） and focal adhesion kinase （FAK） in colorectal cancer （CRC）. Methods Sixty pairs of CRC and adjacent colorectal tissues were collected. The expression of FAK was detected by immunohistochemistry and the expression of miRNA-7 was determined by quantitative real-time polymerase chain reaction （PCR）. Chi square test was used for statistical analysis and Spearman rank was applied for correlation analysis. Results The positive rate of FAK expression in CRC was 75.0% （45/60） and that in adjacent coloreetal tissues was 26.7% （16/60）, the difference was statistically significant （χ^2 = 28. 04, P〈0.01）. The positive rate of phospho-FAK （p-FAK） expression in CRC was 65. 0% （39/60） and that in adjacent colorectal tissues was 21.7 % （13/60）, and the difference was statistically significant （χ^2 = 22.94, P〈0.01）. The expression of miRNA-7 in CRC tissues was down-regulated compared with that in adjacent colorectal tissues （P=0. 044）. The correlation between miRNA-7 expression and lymph nodes metastasis was negative in patients with CRC （Z= -2. 290, P=0. 022）. The miRNA-7 expression was significantly negatively correlated with TNM stage in patients with CRC （Z=2. 698, P=0. 007）. However it was not correlated with age, gender, the location of tumor and tumor differentiation. The correlation between miRNA-7 expression and FAK, p-FAK was negative （Z= -0. 303, P=0. 019;Z=-0. 267, P=0. 038）. Conclusion The miRNA-7 may involved in the genesis and development of CRC through regulating the expression of FAK, which provide a new target for the diagnosis and treatment of CRC.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2015年第8期522-525,共4页
Chinese Journal of Digestion
基金
江西省自然科学基金（20142BAB205054）
江西省研究生创新基金（YC2011-B007）
关键词
结直肠肿瘤
微RNA-7
黏着斑激酶
临床病理特征
Coloreetal neoplasms
MieroRNA-7
Focal adhesion kinase
Clinical pathologic features
