摘要
研究须根在自然腐解的条件下对白花丹参产量及其活性成分含量的影响,利用HPLC测定丹参中活性成分的含量。结果表明,须根腐解使丹参的单株平均产量分别降低了38.60%(20 g),30.99%(40 g);少量的须根(20 g)腐解能够使丹参中二氢丹参酮、隐丹参酮的含量提高26.08%,22.64%;而40 g的须根腐解则显著降低丹参中二氢丹参酮、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA的含量,与对照相比,分别降低60.87%,79.24%,84.61%,88.99%,脂溶性成分总含量降低86.27%。不同浓度的须根腐解能够使丹参中丹酚酸B含量显著提高4.98%(20 g),23.64%(40 g);迷迭香酸的含量分别提高62.44%(20 g),119%(40 g),须根腐解量与水溶性成分含量呈显著正相关。研究结果表明,连作条件下残留的须根对丹参的生长和活性成分的含量有显著的影响,须根腐解能够显著降低丹参的生物量及脂溶性成分的含量,可能为丹参连作障碍形成的重要因素。
The change of yield and contents of active compositions were studied while the fibrous roots were decayed naturally. HPLC method was used to detect the contents of active composition. The results show that fibrousroots could decrease the production of plant by 38.60% （20 g） and 30. 99%（40 g） , respectively. Treatment 1 could increase the contents of dihydrotanshinone and crypto- tanshinone of Salvia miltiorrhiza f. alba by 26. 08% and 22. 64% , respectively. Compared with the comparison, treatment 2 decreased the contents of ihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone Ⅱ A of S. miltiorrhiza f. alba by 60. 87% , 79. 24% , 84. 61% and 88.99% , respectively. Meanwhile, the total contents of the liposoluble constituents reduced by 86. 27%. The different concentration of fibrousroots could increase the content of salvianolic acid B by 4. 98% （20 g） and 23.64% （40 g） , respectively. Meanwhile, the content of rosemary acid was increased by 4. 98% （20 g） and 23.64% （40 g） , respectively. The content of water- soluble constituents positively correlated to the mount of added fibrousroots, and the change was significantly. The result indicted that the decay of fibrousroots has a significant impact on the growth and the content of the active composition of S. miltiorrhiza f. alba under the condition of continuous cropping. Fibrousroots could decrease the content of biomass and liposoluhle constituents significantly, which maybe one of the main factors to S. miltiorrhiza f. alba continuous cropping obstacle formation.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第13期2548-2552,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81102749
81303161)
山东省科技发展计划项目(2012GSF11901)
关键词
白花丹参
须根腐解
产量
活性成分含量
Salvia miltiorrhiza f. alba
fibrousroots decay
yield
content of active composition
