摘要
目的:研究石膏水煎液对肝微粒体细胞色素P450酶CYP1A2和CYP2E1活性的影响,指导临床合理用药。方法:采用双抗体夹心酶联免疫吸附试验(ELISA)对肝脏细胞色素P450酶CYP1A2和CYP2E1进行了初步研究。结果:与空白对照组比较,石膏水煎液对大鼠肝脏中的CYP1A2含量有降低的趋势,对CYP2E1的含量没有显著影响。结论:石膏对大鼠肝微粒体CYP1A2活性有抑制作用,临床上当与经过CYP1A2代谢的药物合用时应适当调整药量。
Objective： To study the influence of Gypsum water decoction on the activity of CYP1 A2 and CYP2E1 of the liver microsomal cytochrome P450,and to guide clinically rational medicine use.Methods：Use a preliminary study with double antibody sandwich enzyme -linked immunosorbent assay （ ELISA ） for CYP1A2 and CYP2E1 of cytochrome P450.Results： Compared with the blank control group, the effect of Gypsum water decoction on the content of CY1A2 in the liver of rats had a reducced tendency,but it had no significant influence on the content of CYP2E1.Conclusion： Gypsum water decoction has an inhibitory effect on the activity of CYP1 A2 , and doctors should adjust the dosage when it is used with medicine which is after CYP1A2 metabolism at the same time.
出处
《中医药信息》
2015年第4期25-28,共4页
Information on Traditional Chinese Medicine
基金
黑龙江省自然科学基金面上项目(No.H201473)
黑龙江省博士后资助课题(No.LBH-Z12259)
黑龙江省教育厅科研课题(No.12531622)
黑龙江中医药大学优秀青年教师支持计划项目(No.2012RCQ16)
